Q: Does viral IL-10 cross-react in bioassay?

A: In an MC9 bioassay, recombinant EBV IL-10 is 20 times less active than recombinant human IL-10.

Recombinant Bovine IL-10 Protein

Novus Biologicals | Catalog # NBP2-62437

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Product Specifications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

Pichia pastoris

Accession Number

P43480

Applications

ELISA

View all IL-10 Proteins and Enzymes »

Product Specifications

Description

Recombinant bovine interleukin-10.

Source: Pichia pastoris

Amino Acid Sequence: 160 amino acid sequence of bovine IL-10, UniProt P43480

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

18.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP2-62437

Preparation Method	Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography.
Formulation	PBS
Preservative	10% Trehalose
Concentration	Lyoph
Reconstitution	Reconstitute with 100 ul sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix vial after reconstitution.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C. After reconstitution store at -20C.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-10

Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Gene Symbol

IL10

UniProt

P43480 (Bovine)

Additional IL-10 Products

Product Documents for Recombinant Bovine IL-10 Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Bovine IL-10 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.