Janelia Fluor® 525, NHS ester
Tocris Bioscience | Catalog # 6296
Key Product Details
Description
Alternative Names
Wavelength
Product Description
Key information:Janelia Fluor® 525, NHS ester is a yellow fluorescent dye; supplied with an NHS ester reactive for the labeling of primary amines. Suitable for live cell imaging.
Application: Suitable for confocal microscopy and super resolution microscopy (SRM) techniques, including dSTORM (in both live and fixed cells). Janelia Fluor® 525, NHS ester is cell permeable.
Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Can be multiplexed for two color imaging with Janelia Fluor® 635, NHS ester (Cat. No. 6419). Excitation and emission maxima (λ) are 525 nm and 549 nm, respectively; quantum yield = 0.91; extinction coefficient = 122,000 M-1cm-1; A280 correction factor = 0.185; lactone-zwitterion equilibrium constant (KL-Z) = 0.068.
Please see the protocol for further information on protein/antibody labeling and conjugation.
We also offer custom conjugation antibody services.
HaloTag® is a registered trademark of Promega Corporation, and SNAP-tag® is a registered trademark of New England BioLabs, Inc.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
This product has been recently renamed. The previous name for this product was Janelia Fluor® 525, SE.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Yellow |
| Brightness | |
| λabs | 525 nm |
| λem | 549 nm |
| Extinction Coefficient (ε) | 122,000 M-1cm-1 |
| Quantum Yield (φ) | 0.91 |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.185 |
| Application | Confocal Microscopy, Super Resolution Microscopy (SRM) |
| Alternative For | Alexa Fluor® 532, Alexa Fluor® 514, Atto 532, CF514, CF532 |
Trademarks and registered trademarks are the property of their respective owners.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Product Specifications for Janelia Fluor® 525, NHS ester
Molecular Weight
Formula
Storage
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 31.18 | 50 |
Preparing Stock Solutions for Janelia Fluor® 525, NHS ester
The following data is based on the product molecular weight 623.51.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 3.21 mL | 16.04 mL | 32.08 mL |
| 2.5 mM | 0.64 mL | 3.21 mL | 6.42 mL |
| 5 mM | 0.32 mL | 1.60 mL | 3.21 mL |
| 25 mM | 0.06 mL | 0.32 mL | 0.64 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Zheng Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 2019 PMID: 31572787
- Grimm A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat. Methods 2017 PMID: 28869757
Product Documents for Janelia Fluor® 525, NHS ester
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Janelia Fluor® 525, NHS ester
For research use only
Customer Reviews for Janelia Fluor® 525, NHS ester
There are currently no reviews for this product. Be the first to review Janelia Fluor® 525, NHS ester and earn rewards!
Have you used Janelia Fluor® 525, NHS ester?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review