Janelia Fluor® 585, NHS ester

Tocris Bioscience | Catalog # 6418

Orange dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy, and two-photon excitation fluorescence microscopy. Suitable for live cell imaging
Tocris Bioscience

Key Product Details

Description

Orange dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy, and two-photon excitation fluorescence microscopy. Suitable for live cell imaging

Alternative Names

JF 585,NHS

Wavelength

Excitation = 585 nm, Emission = 609 nm
View all Amine-Reactive Janelia Fluor Dyes »

Product Description

Key Information:

Janelia Fluor® 585, NHS ester is an orange fluorogenic fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging.

Applications: Suitable for confocal microscopy, two-photon excitation fluorescence microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Cell permeable.

Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 585 nm and 609 nm, respectively; quantum yield = 0.78; extinction coefficient = 156,000 M-1cm-1 (measured in Ethanol or TFE plus 0.1% TFA); A280 correction factor = 0.099; lactone-zwitterion equilibrium constant (KL-Z) < 0.0001. Exhibits low non-specific background staining.

Please see the protocol for further information on protein/antibody labeling and conjugation.

To measure the absorbance spectrum of this dye we recommend the following solvent: trifluoroethanol plus 0.1% TFA.

We also offer custom conjugation antibody services.

HaloTag® is a registered trademark of Promega Corporation, and SNAP-tag® is a registered trademark of New England BioLabs, Inc.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

This product has been recently renamed. The previous name for this product was Janelia Fluor® 585, SE.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Janelia Fluor<sup>®</sup> 585, NHS ester Dye Spectra
Emission Color Orange
Brightness
λabs 585 nm
λem 609 nm
Extinction Coefficient (ε) 156,000 M-1cm-1
Quantum Yield (φ) 0.78
Closest Laser line 561 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
Correction Factor 280 0.099
Application Confocal Microscopy, SRM, Two-photon Excitation Fluorescence Microscopy
Alternative For Alexa Fluor® 568, Atto Rho12, Atto Rho101, CF594

Trademarks and registered trademarks are the property of their respective owners.

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Product Specifications for Janelia Fluor® 585, NHS ester

Molecular Weight

649.60

Formula

C34H27F4N3O6

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

1-[10-[2-Carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-7-(3,3-difluoro-1-azetidinyl)-9,9-dimethyl-2(9H)-anthracenylidene]-3,3-difluoroazetidinium, inner salt

CAS Number

1811539-88-4

PubChem ID

132470913

InChI Key

GFNMIANIZWSVQR-UHFFFAOYSA-N

SMILES

O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CC(F)(F)C4)C=C3C5(C)C)=C(C=C/6)C5=CC6=[N+]7CC(F)(F)C\7)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 64.96 100

Preparing Stock Solutions for Janelia Fluor® 585, NHS ester

The following data is based on the product molecular weight 649.60.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.54 mL 7.70 mL 15.39 mL
5 mM 0.31 mL 1.54 mL 3.08 mL
10 mM 0.15 mL 0.77 mL 1.54 mL
50 mM 0.03 mL 0.15 mL 0.31 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Janelia Fluor® 585, NHS ester

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Janelia Fluor® 585, NHS ester

For research use only

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