Janelia Fluor® 669, NHS ester

Tocris Bioscience | Catalog # 6420

Far red dye (Reactive group: NHS ester). Application: confocal miocrocopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
Tocris Bioscience

Key Product Details

Description

Far red dye (Reactive group: NHS ester). Application: confocal miocrocopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging

Alternative Names

JF 669,NHS

Wavelength

Excitation = 669 nm, Emission = 682 nm
View all Amine-Reactive Janelia Fluor Dyes »

Product Description

Key Information:

Janelia Fluor® 669, NHS ester is a red fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging.

Application: Suitable for confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Cell permeable.

Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 669 nm and 682 nm, respectively; quantum yield = 0.37; extinction coefficient = 116,000 M-1cm-1; A280 correction factor = 0.043. Retains 97% fluorescence after 30 bleaching cycles.

Please see the protocol for further information on protein/antibody labeling and conjugation.

We also offer custom conjugation antibody services.

HaloTag® is a registered trademark of Promega Corporation, and SNAP-tag® is a registered trademark of New England BioLabs, Inc.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

This product has been recently renamed. The previous name for this product was Janelia Fluor® 669, SE.

Scientific Data Images for Janelia Fluor® 669, NHS ester

Applications on Tubulin for Janelia Fluor® 669, NHS ester

Applications on Tubulin for Janelia Fluor® 669, NHS ester.

Application of Janelia Fluor® 669, NHS ester dye on Tubulin; Left: Microtubules were labeled with anti-alpha -tubulin primary antibodies. The secondary antibodies were conjugated to Janelia Fluor® 669, NHS ester. Maximum intensity projection of confocal z-stack, recorded with an Olympus FV1200 confocal microscope, using 638 nm laser line for JF 669. Right: Enlarged portion of left-hand image. Images kindly provided by Dr Kirstin Elgass, Monash University.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Janelia Fluor<sup>®</sup> 669, NHS ester Dye Spectra
Emission Color Far Red
Brightness
λabs 669 nm
λem 682 nm
Extinction Coefficient (ε) 116,000 M-1cm-1
Quantum Yield (φ) 0.37
Closest Laser line 640 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
Correction Factor 280 0.043
Application Confocal Microscopy, Super Resolution Microscopy (SRM)
Alternative For Alexa Fluor® 660, Atto 655, Atto 665, CF660R

Trademarks and registered trademarks are the property of their respective owners.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Product Specifications for Janelia Fluor® 669, NHS ester

Molecular Weight

693.77

Formula

C34H30F3N3O6SSi

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

1-[7-(1-Azetidinyl)-10-[2-Carboxy-5-[(2-(2,5-dioxopyrrolidin-1-yl)oxy)-2-oxoethyl]thio-3,4,6-trifluorophenyl]-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium, inner salt

CAS Number

2127150-20-1

PubChem ID

132470916

InChI Key

QFHKALIQFJQJKE-UHFFFAOYSA-N

SMILES

C[Si](C1=CC(N2CCC2)=CC=C1C(C3=C(F)C(SCC(ON4C(CCC4=O)=O)=O)=C(F)C(F)=C3C([O-])=O)=C5C=C/6)(C)C5=CC6=[N+]7CCC\7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 13.88 20

Preparing Stock Solutions for Janelia Fluor® 669, NHS ester

The following data is based on the product molecular weight 693.77.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 7.21 mL 36.03 mL 72.07 mL
1 mM 1.44 mL 7.21 mL 14.41 mL
2 mM 0.72 mL 3.60 mL 7.21 mL
10 mM 0.14 mL 0.72 mL 1.44 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 5 publications citing the usage of this product.

Product Documents for Janelia Fluor® 669, NHS ester

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Janelia Fluor® 669, NHS ester

For research use only

Citations for Janelia Fluor® 669, NHS ester

Customer Reviews for Janelia Fluor® 669, NHS ester

There are currently no reviews for this product. Be the first to review Janelia Fluor® 669, NHS ester and earn rewards!

Have you used Janelia Fluor® 669, NHS ester?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card