Melan-A/MART-1 Antibody (rMLANA/9404)

Novus Biologicals | Catalog # NBP3-23643

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rMLANA/9404
View all Melan-A/MART-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Melan-A/MART-1 protein

Localization

Cytoplasm.

Marker

Melanoma Marker

Specificity

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for Melan-A/MART-1 Antibody (rMLANA/9404)

Melan-A/MART-1 Antibody (rMLANA/9404) Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (rMLANA/9404) [NBP3-23643] -

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (rMLANA/9404) [NBP3-23643] -

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (rMLANA/9404) [NBP3-23643] - Formalin-fixed, paraffin-embedded human skin stained with Melan-A/MART-1 Antibody (rMLANA/9404). Inset: PBS instead of primary antibody; secondary only negative control.
Melan-A/MART-1 Antibody (rMLANA/9404) Western Blot: Melan-A/MART-1 Antibody (rMLANA/9404) [NBP3-23643] -

Western Blot: Melan-A/MART-1 Antibody (rMLANA/9404) [NBP3-23643] -

Western Blot Analysis of Colo-38 lysate using Melan-A/MART-1 Antibody (rMLANA/9404).

Applications for Melan-A/MART-1 Antibody (rMLANA/9404)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Melan-A/MART-1

MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Alternate Names

Antigen LB39-AA, Antigen SK29-AA, MART-1, MelanA, MLANA

Gene Symbol

MLANA

Additional Melan-A/MART-1 Products

Product Documents for Melan-A/MART-1 Antibody (rMLANA/9404)

Certificate of Analysis

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Product Specific Notices for Melan-A/MART-1 Antibody (rMLANA/9404)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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