Mouse B7-H4 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse B7‑H4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) B7‑1, rmB7‑2, rmB7‑H1, rmB7‑H2, rmB7‑H3, recombinant human (rh) B7‑H3b, rhB7‑H4, or rmPD‑L2 is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 297219
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse B7-H4
    Accession # Q7TSP5
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of B7‑H4 in Mouse Splenoctyes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE‑conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse B7‑H4 APC‑conjugated Monoclonal Antibody (Catalog # FAB2154A) or (B) Rat IgG2B Allophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: B7-H4

B7-H4, also known as VTCN1, B7x and B7S1, is a 50-60 kDa member of the BTN/MOG family of molecules. In mouse, mature B7-H4 is 232 amino acids (aa) in length, and apparently exists as a GPI-linked molecule. This is in contrast to human B7-H4 which is described as being a type I transmembrane protein that also possesses an NLS. There are two V-type Ig-like domains with multiple glycosylation sites. Over aa 29-258, mouse B7-H4 shares 99% and 90% aa sequence indentity with rat and human B7-H4, respectively. Mouse cells known to express B7-H4 include pancreatic islet alpha - and beta -cells, peritoneal macrophages, hepatic stellate cells, dendritic cells and select tumor types. B7-H4 in both mouse and human is known to be solublilized, and to bind to an as-yet unidentified ligand. Although B7-H4 is described as a T cell inactivator, it would appear that it actually fine-tunes an overall downregulation of T cell activity. 

  • Long Name:
    B7 Homolog 4
  • Entrez Gene IDs:
    79679 (Human); 242122 (Mouse)
  • Alternate Names:
    B7h.5; B7H4; B7-H4; B7H4T-cell costimulatory molecule B7x; B7S1; B7S1VCTN1; B7x; B7XPRO1291; FLJ22418; Immune costimulatory protein B7-H4; Protein B7S1; T cell costimulatory molecule B7x; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; Vtcn1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B APC‑conjugated Isotype Control

Ctrl IC013A 3  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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