Detects mouse B7‑H4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) B7‑1, rmB7‑2, rmB7‑H1, rmB7‑H2, rmB7‑H3, recombinant human (rh) B7‑H3b, rhB7‑H4, or rmPD‑L2 is observed.
Monoclonal Rat IgG2B Clone # 297219
Protein A or G purified from hybridoma culture supernatant
Detection of B7‑H4 in Mouse Splenoctyes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE‑conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse B7‑H4 APC‑conjugated Monoclonal Antibody (Catalog # FAB2154A) or (B) Rat IgG2B Allophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
B7-H4, also known as VTCN1, B7x and B7S1, is a 50-60 kDa member of the BTN/MOG family of molecules. In mouse, mature B7-H4 is 232 amino acids (aa) in length, and apparently exists as a GPI-linked molecule. This is in contrast to human B7-H4 which is described as being a type I transmembrane protein that also possesses an NLS. There are two V-type Ig-like domains with multiple glycosylation sites. Over aa 29-258, mouse B7-H4 shares 99% and 90% aa sequence indentity with rat and human B7-H4, respectively. Mouse cells known to express B7-H4 include pancreatic islet alpha - and beta -cells, peritoneal macrophages, hepatic stellate cells, dendritic cells and select tumor types. B7-H4 in both mouse and human is known to be solublilized, and to bind to an as-yet unidentified ligand. Although B7-H4 is described as a T cell inactivator, it would appear that it actually fine-tunes an overall downregulation of T cell activity.
B7 Homolog 4
Entrez Gene IDs:
79679 (Human); 242122 (Mouse)
B7h.5; B7H4; B7-H4; B7H4T-cell costimulatory molecule B7x; B7S1; B7S1VCTN1; B7x; B7XPRO1291; FLJ22418; Immune costimulatory protein B7-H4; Protein B7S1; T cell costimulatory molecule B7x; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; Vtcn1
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