|Detection of B7‑H4 in Mouse Splenoctyes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE‑conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse B7‑H4 APC‑conjugated Monoclonal Antibody (Catalog # FAB2154A) or (B) Rat IgG2B Allophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.|
B7-H4, also known as VTCN1, B7x and B7S1, is a 50-60 kDa member of the BTN/MOG family of molecules. In mouse, mature B7-H4 is 232 amino acids (aa) in length, and apparently exists as a GPI-linked molecule. This is in contrast to human B7-H4 which is described as being a type I transmembrane protein that also possesses an NLS. There are two V-type Ig-like domains with multiple glycosylation sites. Over aa 29-258, mouse B7-H4 shares 99% and 90% aa sequence indentity with rat and human B7-H4, respectively. Mouse cells known to express B7-H4 include pancreatic islet alpha - and beta -cells, peritoneal macrophages, hepatic stellate cells, dendritic cells and select tumor types. B7-H4 in both mouse and human is known to be solublilized, and to bind to an as-yet unidentified ligand. Although B7-H4 is described as a T cell inactivator, it would appear that it actually fine-tunes an overall downregulation of T cell activity.
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