Mouse BAFF R/TNFRSF13C Fluorescein-conjugated Antibody

Discontinued Product

FAB1755F has been discontinued.
Detection of BAFF R/TNFRSF13C in Mouse Splenocytes by Flow Cytometry.
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Mouse BAFF R/TNFRSF13C Fluorescein-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse BAFF R/TNFRSF13C in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) BAFF R is observed, approximately 10‑25% cross-reactivity with recombinant mouse (rm) 4‑1BB, rmDR3, rhDR6, rmEDAR, rhHVEM, rmNGF R, rmOX40, and rmTROY is observed, and no cross-reactivity with rmCD27, rmCD30, rmCD40, rmFAS, rmGITR, rmLymphotoxin  beta R, rmOPG, rmRANK, rmTNFRI, or rmTNF RII is observed.
Source
Monoclonal Rat IgG2A Clone # 204406
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse BAFF R/TNFRSF13C
Gly2-Ala71
Accession # Q9D8D0
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of BAFF R/TNFRSF13C in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of BAFF R/TNFRSF13C in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse BAFF R/TNFRSF13C Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1755F) or (B) Rat IgG2A Fluorescein Isotype Control (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: BAFF R/TNFRSF13C

B-cell activating factor (BAFF), also known as BlyS, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. It is produced by macrophages, dendritic cells, and T lymphocytes. BAFF promotes the survival of B cells and is essential for B cell maturation (1‑4). BAFF binds to three TNF receptor superfamily members: B‑cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL(a proliferation-inducing ligand), BAFF-R selectively binds BAFF. Mouse BAFF-R cDNA encodes a 175 amino acid residue (aa) transmembrane protein with a 71 aa extracellular domain, a 21 aa transmembrane domain, and a 83 aa cytoplasmic region. A second isoform of BAFF R that has a 72 aa cytoplamic region can also be produced by alternative splicing. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cells, in resting CD4+ T cells, in thymus and peripheral blood leukocytes. BAFF knockout mice lack mature B cells and has profound defects in antibody mediated immune responses. Similarly, A/WySnJ mice that are defective in BAFF R intracellular signaling also lack mature B cells, suggesting that BAFF R is the critical receptor for BAFF during B lymphopoiesis. In contrast, BCMA- or TACI-deficient mice have no major defect in B‑cell development. While the function of BCMA is not defined, TACI has been shown to control B‑cell homeostasis and T-cell-independent immune responses.

References
  1. Rolink, A.G. and F. Melcher (2002) Curr. Opin. Immunol. 14:266.
  2. Mackay, F. and J.L. Browning (2002) Nature Reviews Immunology 2:464.
  3. Laabi, Y. et al. (2001) Current Biol. 11:R1013.
  4. Thompson, J.S. et al. (2001) Science 14:2108.
Long Name
B cell Activating Factor Receptor
Entrez Gene IDs
115650 (Human); 72049 (Mouse)
Alternate Names
BAFF R; BAFFR; BR3; CD268; TNFRSF13C

Product Datasheets

Citation for Mouse BAFF R/TNFRSF13C Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. BAFF controls B cell metabolic fitness through a PKC beta- and Akt-dependent mechanism.
    Authors: Patke A, Mecklenbrauker I, Erdjument-Bromage H, Tempst P, Tarakhovsky A
    J. Exp. Med., 2006;203(11):2551-62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow

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