Mouse BID Antibody

(5 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse BID in Western blots. Detects full-length mouse BID and the 15 kDa carboxyl-terminal fragment (tBID) generated by cleavage with Caspase-8. The antibody also detects human BID. However, in Western blots of recombinant human and mouse BID, the antibody is most sensitive for mouse BID.
  • Source
    Monoclonal Rat IgG1 Clone # 91508
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse BID
    Met1-Asp195
    Accession # P70444
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse BID by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 μM staurosporin for 3 hours. PVDF membrane was probed with 1 µg/mL Rat Anti-Mouse BID Monoclonal Antibody (Catalog # MAB860) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands for BID were detected at approximately 12 kDa and 20 kDa (as indicated). For additional reference, a longer exposure is also shown (right panel). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BID
BID is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. BID is cytosolic in healthy cells but upon delivery of an apoptotic signal, BID is cleaved by Caspase-8. The cleaved form is translocated to the mitochondria outer membrane where it binds to BAK and the resulting complex causes altered mitochondrial membrane permeability.
  • Long Name:
    BH3 Interacting Domain Death Agonist
  • Entrez Gene IDs:
    637 (Human); 12122 (Mouse)
  • Alternate Names:
    apoptic death agonist; BH3 interacting domain death agonist; BH3-interacting domain death agonist; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; BID; desmocollin type 4; FP497; Human BID coding sequence; MGC15319; MGC42355; p22 BID
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Sensing cytosolic RpsL by macrophages induces lysosomal cell death and termination of bacterial infection.
    Authors: Zhu, Wenhan, Tao, Lili, Quick, Marsha L, Joyce, Johanna, Qu, Jie-Ming, Luo, Zhao-Qin
    PLoS Pathog, 2015;11(3):e1004704.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. The PI3K regulatory subunits p55alpha and p50alpha regulate cell death in vivo.
    Authors: Pensa S, Neoh K, Resemann H, Kreuzaler P, Abell K, Clarke N, Reinheckel T, Kahn C, Watson C
    Cell Death Differ, 2014;21(9):1442-50.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. BH3-only protein Bid is dispensable for seizure-induced neuronal death and the associated nuclear accumulation of apoptosis-inducing factor.
    Authors: Engel T, Caballero-Caballero A, Schindler CK, Plesnila N, Strasser A, Prehn JH, Henshall DC
    J. Neurochem., 2010;115(1):92-101.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. Activation of the extrinsic caspase pathway in cultured cortical neurons requires p53-mediated down-regulation of the X-linked inhibitor of apoptosis protein to induce apoptosis.
    Authors: Tun C, Guo W, Nguyen H, Yun B, Libby RT, Morrison RS, Garden GA
    J. Neurochem., 2007;102(4):1206-19.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. Proteolysis of HIP during apoptosis occurs within a region similar to the BID loop.
    Authors: Caruso JA, Reiners JJ
    Apoptosis, 2006;11(11):1877-85.
    Species: Mouse
    Sample Type: Recombinant Protein
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 Isotype Control

Ctrl MAB005 37  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG1 Isotype Control

Ctrl MAB005R
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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Average Rating: 5 (Based on 1 review)

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Mouse BID Antibody MAB860
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 WB Anonymous 05/19/2017
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Western Blot Mouse BID Antibody MAB860
Western Blot: Mouse BID Antibody [MAB860]

Summary

ApplicationWestern Blot
Sample TestedMouse embryonic fibroblasts treated with TNFa (50 ng/ml) and cycloheximide (5 ug/ml) for 8 hours

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