Mouse CCR9 Fluorescein-conjugated Antibody

FAB2160F 1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB2160F-025
FAB2160F-100

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Detection of CCR9 in Mouse Thymocytes by Flow Cytometry.

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Citations (1)

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Mouse CCR9 Fluorescein-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCR9 in flow cytometry. Stains mouse CCR9-transfected cells but not irrelevant transfectants.

Source

Monoclonal Rat IgG_2B Clone # 242503

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Y3 rat myeloid cell line transfected with mouse CCR9
Met1-Leu369
Accession # Q9WUT7

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of CCR9 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse CCR9 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2160F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR9

CCR9 is a G protein-linked seven transmembrane domain chemokine receptor that serves as a receptor for CCL25/TECK. CCR9 is expressed on mature and immature thymocytes and some peripheral T and B cells.

Entrez Gene IDs

10803 (Human); 12769 (Mouse); 282832 (Rat)

Alternate Names

C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR9; CCR-9; CD199; CDw199 antigen; CDw199; chemokine (C-C motif) receptor 9; G protein-coupled receptor 28; GPR28; GPR-9-6G-protein coupled receptor 28

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse CCR9 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Functional yet balanced reactivity to Candida albicans requires TRIF, MyD88, and IDO-dependent inhibition of Rorc.
Authors: De Luca A, Montagnoli C, Zelante T, Bonifazi P, Bozza S, Moretti S, D'Angelo C, Vacca C, Boon L, Bistoni F, Puccetti P, Fallarino F, Romani L
J. Immunol., 2007;179(9):5999-6008.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry