Mouse CD11b/Integrin alpha M Antibody

Catalog # Availability Size / Price Qty
MAB11241-100
MAB11241-SP
Detection  of Mouse CD11b/Integrin alpha M by Western Blot.
2 Images
Product Details
Citations (1)
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Mouse CD11b/Integrin alpha M Antibody Summary

Species Reactivity
Mouse
Specificity
Detects recombinant mouse CD11b/Integrin alpha M in Western blot.
Source
Monoclonal Rat IgG2B Clone # 908649
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha M beta 2
Phe17-Asn1105 (Integrin alpha M), Gln24-Asn702 (Integrin beta 2)
Accession # NP_032427 (Integrin alpha M) & P11835 (Integrin beta 2)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Mouse CD11b/Integrin alpha M by Western Blot. View Larger

Detection of Mouse CD11b/Integrin alpha M by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Mouse CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB11241) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD11b/Integrin alpha M at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection  of CD11b/Integrin alpha M in RAW 264.7 Mouse Cell Line by Flow Cytometry View Larger

Detection of CD11b/Integrin alpha M in RAW 264.7 Mouse Cell Line by Flow Cytometry RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB11241, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha  and a beta  subunit. The Integrin  alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin  beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin  alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin  alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles (1,2).

References
  1. Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
  2. Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.
Entrez Gene IDs
3684 (Human); 16409 (Mouse)
Alternate Names
antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide); Cell surface glycoprotein MAC-1 subunit alpha; CR-3 alpha chain; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6

Product Datasheets

Citation for Mouse CD11b/Integrin alpha M Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Diet-dependent function of the extracellular matrix proteoglycan Lumican in obesity and glucose homeostasis
    Authors: G Wolff, AE Taranko, I Meln, J Weinmann, T Sijmonsma, S Lerch, D Heide, AT Billeter, D Tews, D Krunic, P Fischer-Po, BP Müller-Sti, S Herzig, D Grimm, M Heikenwäld, WW Kao, A Vegiopoulo
    Mol Metab, 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenate
    Applications: Western Blot

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