|Detection of CD161/NK1.1 in C57BL/6 Mouse Splenocytes by Flow Cytometry. C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse NKp46/NCR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB22252P) and either (A) Mouse Anti-Mouse CD161/NK1.1 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB8319N) or (B) Mouse IgG2A Alexa Fluor 700 Isotype Control (Catalog # IC003N). View our protocol for Staining Membrane-associated Proteins.|
CD161, also known as NK1.1, KLRB-1C, Ly-55, and NKR-P1c, is a 28 kDa type 2 transmembrane protein in the Killer Cell Lectin-like Receptor family. CD161 is expressed as a disulfide-linked homodimer on the surface of NK cells and subpopulations of NKT, CD4+, CD8+, and gamma /δ T cells. Its cross-linking on NK cells induces cytolytic activity, and on CD4 cells it promotes IgE production and the expansion of Th2 responses. Mature mouse CD161 consists of a 45 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 157 aa extracellular domain with one C-type lectin domain.
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