Detection of CD200 R3 in MC/9‑2 Mouse Cell Line by Flow Cytometry.
MC/9‑2 mouse mast cell line was stained with Sheep Anti-Mouse CD200 R3 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6554P, filled histogram) or isotype control antibody (Catalog # IC016P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD200 R3
Mouse CD200 R3, also known as CD200RLb, is a 38-40 kDa type I transmembrane glycoprotein member of a small family of CD200R-related molecules. Family members named CD200R1-5 represent activating counterparts to CD200R that is a widely expressed inhibitory receptor for the ligand CD200. Consistent with other family members, CD200 R3 is a Ig-superfamily member with a V-type and C2-type Ig-like domain. But unlike other family members, it presents as a disulfide-linked homodimer that is highly spliced, poorly glycosylated, and shows an unusual modification of its C2-type Ig-like domain. It is expressed by mast cells, basophils, and CD49+ regulatory dendritic cells. It does not bind CD200. When experimentally activated, it induces IL-4 production and degranulation. There is no direct human or rat counterpart to mouse CD200 R3.
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