Mouse CD28 Antibody

Catalog # Availability Size / Price Qty
    Mouse CD28  Antibody Induces Proliferation in Mouse T Cells.
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Product Details
Citations (4)
Supplemental Products

Mouse CD28 Antibody Summary

Species Reactivity
Detects mouse CD28 in ELISA.
Monoclonal Rat IgG1 Clone # 794716
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse CD28
Accession # P31041
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Flow Cytometry
0.25 µg/106 cells
See below
Agonist Activity
0.07-0.42 µg/mL
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Agonist Activity Mouse CD28  Antibody Induces Proliferation in Mouse T Cells. View Larger

Mouse CD28 Antibody Induces Proliferation in Mouse T Cells. Rat Anti-Mouse CD28 Monoclonal Antibody (Catalog # MAB4832) induces proliferation in mouse T cells in the presence of 100 ng/mL Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484), in a dose dependent manner, as measured by Resazurin (Catalog # AR002), The ED50 for this effect is typically 0.07-0.42 μg/mL.

Flow Cytometry Detection of CD28 in CD3+ Mouse Splenocytes by Flow Cytometry. View Larger

Detection of CD28 in CD3+ Mouse Splenocytes by Flow Cytometry. CD3+ mouse splenocytes were stained with Rat Anti-Mouse CD28 Monoclonal Antibody (Catalog # MAB4832, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD28

CD28 and CTLA-4, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig
V‑like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is down-regulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is upregulated rapidly following T cell activation and CD28 ligation. Cell surface expression of CTLA-4 peaks approximately 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to
B7‑1 and B7‑2 with a 20-100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the up-regulation of
Bcl‑xL. CD28 ligation has also been shown to regulate Th1/Th2 differentiation. Agonist activity has been reported using MAB4831 (4,5).

  1. Lenschow, D.J. et al. (1996) Annu. Rev. Immunol. 14:233.
  2. Hathcock, K.S. and R.J. Hodes (1996) Advances in Immunol. 62:131.
  3. Ward, S.G. (1996) Biochem. J. 318:361.
  4. Nguyen, P. et al. (2003) Blood 13:4320.
  5. Orbach, A. et al. (2007) J. Immunol. 179:7287.
Entrez Gene IDs
940 (Human); 12487 (Mouse); 102146670 (Cynomolgus Monkey)
Alternate Names
CD28 antigen (Tp44); CD28 antigen; CD28 molecule; CD28; MGC138290; T-cell-specific surface glycoprotein CD28; Tp44

Product Datasheets

Citations for Mouse CD28 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Crucial role of OX40/OX40L signaling in a murine model of asthma
    Authors: W Lei, D Zeng, G Liu, Y Zhu, J Wang, H Wu, J Jiang, J Huang
    Mol Med Rep, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Func
  2. The excretory-secretory products of Echinococcus granulosus protoscoleces directly regulate the differentiation of B10, B17 and Th17 cells
    Authors: W Pan, WT Hao, YJ Shen, XY Li, YJ Wang, FF Sun, JH Yin, J Zhang, RX Tang, JP Cao, KY Zheng
    Parasit Vectors, 2017;10(1):348.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Func
  3. Aloperine Protects Mice against DSS-Induced Colitis by PP2A-Mediated PI3K/Akt/mTOR Signaling Suppression
    Authors: X Fu, F Sun, F Wang, J Zhang, B Zheng, J Zhong, T Yue, X Zheng, JF Xu, CY Wang
    Mediators Inflamm., 2017;2017(0):5706152.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Func
  4. Temporal Expression of Bim Limits the Development of Agonist-Selected Thymocytes and Skews Their TCR? Repertoire
    Authors: David A Hildeman
    J. Immunol., 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Func


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