Mouse CD36/SR-B3 APC-conjugated Antibody

FAB25191A has been discontinued. View all CD36/SR-B3 products.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD36/SR-B3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CD36 is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 324205
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse CD36/SR-B3
    Gly30-Lys439
    Accession # Q08857
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD36/SR‑B3 in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse CD36/SR‑B3 APC‑conjugated Monoclonal Antibody (Catalog # FAB25191A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD36/SR-B3

CD36 (alternatively known as platelet membrane glycoprotein IV (GPIV), thrombospondin receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3)) is an 88 kDa, integral membrane glycoprotein that belongs to the class B scavenger receptor family (1, 2). The molecule is described as being ditopic, with two transmembrane segments connected by an extracellular loop (3). Mouse CD36 is synthesized as a 472 amino acid (aa) protein that contains a 6 aa N‑terminal cytoplasmic domain, a 22 aa N‑terminal transmembrane segment, a 420 aa extracellular “loop”, a 22 aa C‑terminal transmembrane segment, and a 9 aa C‑terminal cytoplasmic tail (4). Both cytoplasmic tails are palmitoylated, with the C‑terminal tail involved in oxidized LDL binding (5, 6). With respect to the extracellular loop, the N‑terminal region is believed to bind both thrombospondin-1 and Plasmodium-infected erythrocytes. Other ligands for CD36 include long-chain fatty acids, collagen, phospholipids and apoptotic cells (1). The extracellular loop of mouse CD36 shares 94%, 92%, 84% and 84% aa sequence identity with the extracellular loops of rat, hamster, human and bovine CD36, respectively. Cells known to express CD36 include capillary endothelium, adipocytes, skeletal muscle cells, intestinal epithelium, smooth muscle cells and hematopoietic cells such as RBC’s, platelets and monocytes (1). On the surface of cells, CD36 is suggested to exist as a dimer in response to ligation (7). CD36 is reported to regulate fatty uptake, act as an angiogenic with TSP-1, and participate in the clearance of apoptotic phagocytes (1, 8).

  • References:
    1. Febbraio, M. et al. (2001) J. Clin. Invest. 108:795. 
    2. Silverstein, R.L. and M. Febbraio (2000) Curr. Opin. Lipid. 11:483. 
    3. Gruarin, P. et al. (2000) Biochem. Biophys. Res. Commun. 275:446. 
    4. Endemann, G. et al. (1993) J. Biol. Chem. 268:11811. 
    5. Malaud, E. et al. (2002) Biochem. J. 364:507.
    6. Tao, N. et al. (1996) J. Biol. Chem. 271:22315.
    7. Daviet, L. et al. (1997) Thromb. Haemost. 78:897.
    8. Simantov, R. and R.L. Silverstein (2003) Front. Biosci. 8:s874.
  • Long Name:
    Scavenger Receptor Class B, Member 3
  • Entrez Gene IDs:
    948 (Human); 12491 (Mouse)
  • Alternate Names:
    CD36 antigen; CD36 molecule (thrombospondin receptor); CD36; Collagen R; FAT; FATCHDS7; Fatty acid translocase; Glycoprotein IIIb; GP3Bthrombospondin receptor); GPIIIb; GPIV; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; Platelet collagen receptor; platelet glycoprotein 4; Platelet glycoprotein IV; SCARB3; scavenger receptor class B, member 3; SRB3; SR-B3; Thrombospondin R; Thrombospondin receptor
Related Research Areas

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