Mouse CD39/ENTPD1 PE-conjugated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD39/ENTPD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human CD39 is observed and no cross-reactivity with recombinant mouse CD39L3 or recombinant human CD39L4 is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 495826
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD39/ENTPD1
    Thr38-Ile478
    Accession # AAH11278
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD39/ENTPD1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD39/ENTPD1 PE‑conjugated Monoclonal Antibody (Catalog # FAB4398P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD39/ENTPD1

Ectonucleoside Triphosphate Diphosphohydrolase-1 (ENTPD1) is a 65-80 kDa, two-transmembrane glycoprotein that contains an N- and C-terminal cytoplasmic domain (1, 2). ENTPD1 was originally described as CD39, a B lymphocyte cell surface marker and is now known to also be present on the surface of endothelial cells, neutrophils, mast cells, macrophages, pancreatic acinar and duct cells, dendritic cells, sympathetic neuron axon terminals, CD4+ CD39+ CD161+ Th17 precursors, PD-1+ CD39+ "exhausted" CD8+ T cells and FoxP3+ CD25+ CD4+ CD127- CD49d- suppressor plus FoxP3- CD25- CD4+ CD127+ CD49d+ non-suppressor T cells (1-9). ENTPD1 hydrolyzes the beta - and gamma  phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, ENTPD1 plays a role in the regulation of purinergic signaling. Extracellular ATP released from dead or stressed cell creates a proinflammatory environment. In concert with CD73, the conversion of ATP to adenosine reverses this and creates an antiinflammatory environment (1,2). Over amino acids (aa) 38-478, mouse ENTPD1 shares 90% and 76% aa sequence identity with rat and human ENTPD1, respectively

  • References:
    1. Bono, M.R. et al. (2015) FEBS Lett. 589:3454.
    2. Antonioli, L. et al. (2013) Trends Mol. Med. 19:355.
    3. Machida, T. et al. (2005) J. Pharmacol. Exp. Ther. 313:570.
    4. Aldi, S. et al. (2015) FASEB J. 29:61.
    5. Schuler, P.J. et al. (2011) J. Immunol. Methods 369:59.
    6. Gupta, P.K. et al. (2015) PLoS Pathog. 11:e1005177.
    7. Bai, A. and S. Robson (2015) Purinergic Signal. 11:317.
    8. Sorenson, C.E. et al. (2003) J. Physiol. 551:881.
    9. Mascanfroni, I.D. et al. (2013) Nat. Immunol. 14:1054.
  • Long Name:
    Ecto-Nucleoside Triphosphate Diphosphohydrolase 1
  • Entrez Gene IDs:
    953 (Human); 12495 (Mouse); 64519 (Rat)
  • Alternate Names:
    ADPase; ATPDase; ATP-diphosphatase; CD39 antigen; CD39; CD39EC 3.6.1.5; DKFZp686D194; DKFZp686I093; EC 3.6.1; ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; ectonucleoside triphosphate diphosphohydrolase 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPD1; NTPDase 1; NTPDase-1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 PE-conjugated Antibody

Ctrl IC005P  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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