Detection of CD45.2 in BALB/c Mouse Splenocytes by Flow Cytometry. BALB/c mouse splenocytes were stained with Mouse Anti-Mouse CD45.2 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB8448N, filled histogram) or isotype control antibody (Catalog # IC003N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CD45, previously called LCA (Leukocyte Common Antigen), T200, or Ly5 in mice, is member C of the Class 1 (Receptor-like) Protein Tyrosine Phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1-3). CD45.2 is an alloantigen of CD45 expressed by Ly5.2+ mouse strains including: AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, and 129.
Anderson, J.N. et al. (2004) FASEB J. 18:8.
Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
Cluster of Differentiation 45.2
Entrez Gene IDs:
5788 (Human); 19264 (Mouse)
B220; CD 45; cd45 antigen; CD45.2; CD45R; GP 180; GP180; Human homolog of severe combined immunodeficiency due to PTPRC deficiency; L CA; LCA; Leukocyte common antigen; LY 5; Ly5; Ly5.2; Ly-5.2; Protein tyrosine phosphatase receptor type c polypeptide; Protein tyrosine phosphatase receptor type C; PTPRC; RT7.2; SCID due to PTPRC deficiency; T200 glycoprotein; T200 leukocyte common antigen; T200
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