Mouse CD6 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse CD6 in direct ELISAs and Western blots. Does not cross-react with human CD6.
  • Source
    Monoclonal Rat IgG2A Clone # 96123
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD6
    Accession # Q61003
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CD6 PE‑conjugated Monoclonal Antibody (Catalog # FAB727P) or (B) Rat IgG2A Phycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD6

CD6 is a member of the group B scavenger receptor cysteine-rich (SRCR) superfamily. CD6 is a type I membrane glycoprotein and contains three extracellular SRCR domains. CD6 is expressed at low levels on immature thymocytes and at high levels on mature thymocytes. The majority of peripheral blood T cells, a subset of B cells, and a subset of neuronal cells express CD6. Mouse CD6 is a 626 amino acid (aa) protein with a 24 aa signal sequence, a 372 aa extracellular domain, and a 204 aa cytoplasmic region. The 668 aa human homolog has also been identified. The human and murine proteins share 70% aa identity over their full-lengths.

The role of CD6 has not been fully elucidated. However, it appears to play a role as both a co-stimulatory molecule in T cell activation and as an adhesion receptor. Studies demonstrating a mitogenic effect for T cells with some CD6 specific monoclonal antibodies, in conjunction with either accessory cells or PMA and anti-CD2 mAb, support the concept of CD6 as a co-stimulatory molecule. Additionally, anti-CD6 monoclonal antibody has been used as an immunosuppressive agent for patients undergoing kidney or bone marrow allograft rejection. It has also been used to remove CD6+ T cells from donor bone marrow prior to allogenic bone marrow transplantation. Other studies have demonstrated an adhesive role for CD6, it has been demonstrated to bind the activated leukocyte cell adhesion molecule (ALCAM, CD166). CD6/ALCAM interactions have been postulated to play a role in thymocyte development. Additionally, the presence of ALCAM on neuronal cells may provide a mechanism of interaction between CD6+ T cell and ALCAM+ neuronal cells. Phosphorylation of the CD6 molecule appears to play a role in CD6-mediated signal transduction. Serine and threonine residues become hyperphosphorylated and tyrosine residues become phosphorylated when T cells are activated with anti-CD6 mAb in conjunction with PMA, anti-CD2, or anti-CD3 mAb. The CD6 intracellular domain contains regions that can interact with SH2 or SH3 containing proteins. However, the signaling pathways have not been elucidated.

  • References:
    1. Gangemi, R. et al. (1989) J. Immunol. 143:2439.
    2. Aruffo, A. et al. (1991) J. Exp. Med. 174:949.
    3. Swack, J.A. et al. (1991) J. Biol. Chem. 266:7137.
    4. Robinson, W.H. et al. (1995) Eur. J. Immunol. 25:2765.
    5. Whitney, G. et al. (1995) Mol. Immunol. 32:89.
    6. Starling, G.C. et al. (1996) Eur. J. Immunol. 26:738.
    7. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
    8. Swack, J.A. et al. (1989) Mol. Immunol. 26:1037.
    9. Pawelec, G. and H.J. Buhring (1991) Human Immunol. 31:165.
    10. Osorio, L.M. et al. (1995) Cell Immunol. 166:44.
    11. Robinson, W.H. et al. (1995) J. Immunol. 155:4739.
    12. Singer, N.G. et al. (1996) Immunology 88:537.
    13. Aruffo, A. et al. (1997) Immunol. Today 18:498.
  • Entrez Gene IDs:
    923 (Human); 12511 (Mouse)
  • Alternate Names:
    CD6 antigenFLJ44171; CD6 molecule; CD6; T12; Tp120; TP120T-cell differentiation antigen CD6
Related Research Areas
Isotype Controls
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Rat IgG2A PE-conjugated Antibody

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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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