|Detection of CD63 in HEK293 Human Cell Line Transfected with Mouse CD63 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse CD63 and eGFP was stained with either (A) Rat Anti-Mouse CD63 APC‑conjugated Monoclonal Antibody (Catalog # FAB5417A) or (B) Rat IgG1 Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.|
CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking molecules. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation, is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of mouse CD63 share 94% and 67% amino acid sequence identity with rat and human CD63, respectively.
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