Mouse CD9 PE-conjugated Antibody

FAB5218P has been discontinued. View all CD9 products.
Datasheet / CoA / SDS
Product Details
  • Species Reactivity
  • Specificity
    Stains mouse CD9-transfected cells in flow cytometry,  but not irrelevant transfectants.
  • Source
    Monoclonal Rat IgG2A Clone # 479608
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with mouse CD9
    Accession # P40240
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
Product Datasheets

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  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CD9 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse CD9 PE‑conjugated Monoclonal Antibody (Catalog # FAB5218P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD9

CD9 is a 22-25 kDa member of the tetraspanin (TM4SF) superfamily of molecules. Although typology of this family shows a cytoplasmic N- and C-terminus, tetraspanins should not be confused with the MS4A (Membrane-spanning 4 domain) family of proteins. To date, there are more than 30 human tetraspanins, presumably all of which participate in the creation of tetraspanin webs (or multimolecular microdomains) that traverse the cell membrane. As with other superfamily members, CD9 forms homodimers and associates with essentially five classes of molecules: Integrins ( alpha IIb beta 3, alpha 6 beta 4, alpha 3-6 beta 1), IgSF members (ICAM-1, VCAM-1, IgSF8/EWI-2, CD9P1), ectoenzymes (ADAM-12, MT1-MMP) intracellular signaling moluecules (PI-4K, PKC) and others (CD81, CD63, IL-16, pro-HBEGF). The microdomain complexes both recruit and regulate individual molecules. CD9-containing microdomains reportedly promote gamete fusion while inhibiting monocyte fusion. Cells known to express CD9 are many and varied, and include oocytes, regulatory B cells (in mouse, not human), endothelial cells, platelets, monocytes, keratinocytes, mast cells, and myoblasts. The extracellular domain(s) or mouse CD9 share 80% and 88% amino acid sequence identity with human and rat CD9, respectively.

  • Entrez Gene IDs:
    928 (Human); 12527 (Mouse)
  • Alternate Names:
    BA2; BA-2/p24 antigen; BTCC-1; CD9 antigen; CD9 molecule; CD9; Cell growth-inhibiting gene 2 protein; DRAP-27; Leukocyte antigen MIC3; MIC3; motility related protein-1,5H9 antigen; Motility-related protein; MRP-1FLJ99568; p24; tetraspanin-29; TSPAN29; TSPAN-29; TSPAN29MIC3CD9 antigen (p24)
Related Research Areas


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