Mouse Chordin-like 2/CHRDL2 Antibody

Catalog # Availability Size / Price Qty
MAB2520
MAB2520-SP

15% Off Limited-time Savings on Your Reagents, Assays, and Kits
Now through September 27th, you can save 15% on Bio-Techne reagents, assays, and kits (including Novus, Tocris, and R&D Systems). Use promotion code SAVE15 at checkout on orders over $1,000. See terms and conditions for offer details.

Chordin-like 2/CHRDL2 in Embryonic Mouse Cartilage.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Mouse Chordin-like 2/CHRDL2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Chordin-like 2/CHRDL2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CHRDL2, recombinant mouse (rm) CHRDL1, or rmChordin is observed.
Source
Monoclonal Rat IgG2A Clone # 328905
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Chordin-like 2/CHRDL2
Gln24-Leu426
Accession # Q8VEA6.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Chordin-Like 2/CHRDL2 (Catalog # 2520-CH)
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Chordin-like 2/CHRDL2 (Catalog # 2520-CH), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Chordin-like 2/CHRDL2 antibody in Embryonic Mouse Cartilage by Immunohistochemistry (IHC-Fr). View Larger

Chordin-like 2/CHRDL2 in Embryonic Mouse Cartilage. Chordin-like 2/ CHRDL2 was detected in immersion fixed frozen sections of embryonic mouse cartilage (15 d.p.c.) using 25 µg/mL Rat Anti-Mouse Chordin-like 2/CHRDL2 Monoclonal Antibody (Catalog # MAB2520) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chordin-like 2/CHRDL2

Chordin-like 2 (CHL2; also known as breast tumor novel factor 1/BNF-1), is a secreted glycoprotein that has significant homology to chordin and acts as a TGF-beta superfamily antagonist (1‑3). CHL2 is a chordin family member with structural homology to CHL1 (also known as neuralin or ventroptin) (1, 2). Mouse CHL2401 cDNA encodes a 45‑50 kDa, 426 amino acid (aa) residue precursor protein with a putative 25 aa signal peptide and a 401 aa mature segment. The mature segment contains three 63 aa cysteine-rich von Willebrand type C repeats (CRs) that are conserved among chordin family members in the spacing of 10 cysteine residues (1, 2). It also contains two potential N-linked glycosylation sites and one putative NLS that lies just proximal to the third CR repeat.

Mouse CHL2, like human CHL2, appears to undergo extensive alternate splicing. This splicing generates both secreted and intracellular forms of CHL2, and influences the type of TGF-beta superfamily member bound (1, 2). The CRs of chordin, especially CR1 and CR3, have been shown to be the functional domains for BMP binding (4). The CR1 and CR3 of CHL proteins are most similar to CR3 of chordin (1). Mature mouse and human CHL2 share 71% amino acid identity, while mouse CHL1 and -2 share 40% amino acid identity (2). Like chordin, CHL2401 exhibits BMP inhibitory activity by directly interacting with BMP-4 and preventing binding to its receptor (1). However, another CHL2 isoform with an additional exon 9b has been shown to bind and inhibit Activin A activity as well (2). CHL2401 is not abundantly expressed in mouse embryos, but is detected only in the chondrocytes of developing joints and in the connective tissue of reproductive organs (1). Mouse CHL2 acts to reduce the rate of matrix accumulation in mesenchymal cells, acting as a negative regulator of cartilage formation (1). In the adult mouse, CHL2 is again detected only faintly in liver, kidney, skeletal muscle and testis (1). Expression patterns in human tissue blots are distinct from those expressed in mouse (1, 2).

References
  1. Nakayama, N. et al. (2004) Development 131:229.
  2. Oren, A. et al. (2004) Gene 331:17.
  3. Wu, I. and M.A. Moses (2003) Gene 311:105.
  4. Larrain, J. et al. (2000) Development 127:821.
Entrez Gene IDs
25884 (Human); 69121 (Mouse)
Alternate Names
BNF-1; Chordin like 2; Chordin-like 2; chordin-like protein 2; CHRDL2

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse Chordin-like 2/CHRDL2 Antibody

There are currently no reviews for this product. Be the first to review Mouse Chordin-like 2/CHRDL2 Antibody and earn rewards!

Have you used Mouse Chordin-like 2/CHRDL2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review