Mouse CLEC9a PE-conjugated Antibody

FAB67761P has been discontinued. View all CLEC9a products.
    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CLEC9a in flow cytometry.
  • Source
    Monoclonal Rat IgG1 Clone # 7H11
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    RBL‑2H3 rat basophilic leukemia cell line expressing mouse CLEC9a fused to an HA epitope
    Accession # Q8BRU4
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CLEC9a in Mouse Splenoctyes by Flow Cytometry. CD4- CD8+ mouse splenocytes were stained with Hamster Anti-Mouse CD11c APC‑conjugated Monoclonal Antibody (Catalog # FAB69501A) and either (A) Rat Anti-Mouse CLEC9a PE‑conjugated Monoclonal Antibody (Catalog # FAB67761P) or (B) Rat IgG1 Phycoerythrin Isotype Control (Catalog # IC005P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CLEC9a
CLEC9a (C-type Lectin domain family 9 member A), also known as DNGR-1, is a 44-48 kDa type II transmembrane glycoprotein that belongs to the C-type lectin superfamily. Although the CTLD of CLEC9a structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9a is expressed as either a disulfide-linked homodimer or monomer. Human CLEC9a expression is restricted to a subpopulation of BDCA-3+ conventional dendritic cells (cDC) and CD16- monocytes. In mouse, the human BDCA-3+ cDC are analogous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules. In addition, mouse CLEC9a is also expressed on plasmacytoid dendritic cells. CLEC9a ligation enhances antigen uptake and processing, leading to presentation on MHC class I molecules and cytotoxic T cell (CTL) priming. In mouse, CLEC9a recognizes normally intracellular determinant(s) of necrotic cells and mediates their uptake by the dendritic cell. The subsequent antigenic cross-presentation to CTL is important for clearing necrotic cellular debris. CLEC9a signaling triggers activation of the tyrosine kinase Syk, indicating that this is an activating receptor. Alternative splicing of mouse CLEC9a generates isoforms with deletions in the transmembrane segment, stalk region, or CTLD. Within aa 57-264 of the ECD, mouse CLEC9a shares 57% and 80% aa sequence identity with human and rat CLEC9a, respectively.
  • Long Name:
    C-type Lectin-like Receptor 9
  • Entrez Gene IDs:
    283420 (Human); 232414 (Mouse)
  • Alternate Names:
    CD370; CLEC9a; C-type lectin domain family 9 member A; C-type lectin domain family 9, member A; DNGR1; HEEE9341; UNQ9341
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