Mouse Common  gamma  Chain/IL-2 R gamma APC-conjugated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Common gamma Chain/IL-2 R gamma in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-2 R alpha, rmIL‑2 R beta, recombinant human (rh) IL-2 R gamma, rhIL-15 R alpha, recombinant rat IL-2 R alpha, or recombinant canine IL-2 R alpha is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 644346
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf21-derived recombinant mouse Common gamma Chain/IL-2 R gamma
    Trp23-Ala263 (predicted)
    Accession # P34902
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Common  gamma  Chain/IL‑2 R gamma in Mouse Splenocytes by Flow Cytometry. CD3+ mouse splenocytes were stained with Rat Anti-Mouse Common  gamma  Chain/IL‑2 R gamma APC‑conjugated Monoclonal Antibody (Catalog # FAB7842A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Common gamma Chain/IL-2 R gamma
The Common gamma Chain, also known as CD132, is a transmembrane protein belonging to the hematopoietin receptor family. gamma c is a signaling component of the functional receptor complexes for IL-2, IL-4, IL-7, IL-9, and IL-15.
  • Long Name:
    IL-2 Receptor gamma
  • Entrez Gene IDs:
    3561 (Human); 16186 (Mouse)
  • Alternate Names:
    CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; Common gamma Chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; P64; SCIDX; SCIDX1; severe combined immunodeficiency
Related Research Areas

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