Detects mouse Common gamma Chain/IL-2 R gamma in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-2 R alpha, rmIL‑2 R beta, recombinant human (rh) IL-2 R gamma, rhIL-15 R alpha, recombinant rat IL-2 R alpha, or recombinant canine IL-2 R alpha is observed.
Monoclonal Rat IgG2B Clone # 644346
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf21-derived recombinant mouse Common gamma Chain/IL-2 R gamma Trp23-Ala263 (predicted) Accession # P34902
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Common gamma Chain/IL‑2 R gamma in Mouse Splenocytes by Flow Cytometry. CD3+ mouse splenocytes were stained with Rat Anti-Mouse Common gamma Chain/IL‑2 R gamma APC‑conjugated Monoclonal Antibody (Catalog # FAB7842A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Common gamma Chain/IL-2 R gamma
The Common gamma Chain, also known as CD132, is a transmembrane protein belonging to the hematopoietin receptor family. gamma c is a signaling component of the functional receptor complexes for IL-2, IL-4, IL-7, IL-9, and IL-15.
IL-2 Receptor gamma
Entrez Gene IDs:
3561 (Human); 16186 (Mouse)
CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; P64; SCIDX; SCIDX1; severe combined immunodeficiency
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.