Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Transgenic Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1174A
View all CXCL1/GRO alpha/KC/CINC-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CXCL1/GRO alpha /KC/CINC‑1
Asn29-Lys96
Accession # P12850

Specificity

Detects mouse CXCL1/GRO alpha /KC/CINC‑1 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody

Detection of CXCL1/GROa/KC/CINC‑1 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CXCL1/GRO alpha /KC/CINC‑1 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes either (A) treated with 1 µg/mL LPS and Brefeldin A for 4 hours or (B) untreated were stained with Rabbit Anti-Mouse CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB4532) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) and Rat Anti-Mouse TNF-a Fluorescein-conjugated Monoclonal Antibody (Catalog # IC410F). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Applications for Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25-1 µg/106 cells
Sample: Mouse splenocytes treated with LPS and Brefeldin A were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Reviewed Applications

Read 1 review rated 3 using MAB4532 in the following applications:

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL1/GRO alpha/KC/CINC-1

KC, a member of the alpha (CXC) chemokine subfamily, was initially identified as an immediate early gene induced in mouse fibroblasts by platelet‑derived growth factor. KC cDNA encodes a 96 amino acid (aa) residue precursor protein with a predicted secretory signal peptide that is removed to yield the mature protein. The protein sequence of mouse KC shows approximately 63% identity to that of mouse MIP-2. KC is also approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the orthologs of the human GROs and rat CINCs. In addition to mouse fibroblasts, KC is expressed in macrophages and endothelial cells. Mouse KC is a potent neutrophil attractant and activator. The functional receptor for KC has been identified as CXCR2. Based on the pattern of KC expression in a number of inflammatory disease models, KC appears to have an important role in inflammation. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer’s disease. Many chemokines are substrates for selective proteolysis at the amino-terminus by various proteases including dipeptidyl peptidase IV or matrix metalloproteases, resulting in truncated chemokine isoforms with different (both enhanced or reduced) bioactivities. The naturally occurring 68 aa N-terminal truncated isoform of mouse KC is reported to be a more potent synergistic growth stimulant for CFU-GM.

Alternate Names

CINC-1, CINC1, GRO alpha, KC, MGSA-alpha

Entrez Gene IDs

2919 (Human); 14825 (Mouse); 81503 (Rat)

Gene Symbol

CXCL1

UniProt

P12850

Additional CXCL1/GRO alpha/KC/CINC-1 Products

Product Documents for Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody

For research use only

Citations for Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody

Customer Reviews for Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody (1)

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  • Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: mouse lung total leukocytes
    Species: Mouse
    Verified Customer | Posted 02/26/2016
    Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody MAB4532

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