Mouse CXCR4 PE-conjugated Antibody

FAB21651P 7 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB21651P-025
FAB21651P-100

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Mouse CXCR4 PE-conjugated Antibody in Data

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Citations (7)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCR4 PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CXCR4 transfectants. Does not stain irrelevant transfectants.

Source

Monoclonal Rat IgG_2B Clone # 247506

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Y3 rat myeloid cell line transfected with mouse CXCR4
Met1-Ser359
Accession # P70658

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of CXCR4 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with (A) Rat Anti-Mouse CXCR4 PE-conjugated Monoclonal Antibody (Catalog # FAB21651P) or (B) Rat IgG_2Bisotype control antibody (Catalog # IC013P) and Rat anti-Mouse CD8 alpha APC-conjugated Monoclonal Antibody (Catalog # FAB116A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR4

CXCR4, also known as CD184, is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).

References

Orsini, M.J. et al. (1999) J. Biol. Chem. 274:31076.
Zagzag, D. et al. (2005) Cancer Res. 65:6178.
Speetjens, F.M. et al. (2009) Cancer Microenvironment 2:1.
Wang, L. et al. (2009) Oncology Reports 22:1333.
Amara, S. et al. (2015) Cancer Biomark. 15:869.

Entrez Gene IDs

7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)

Alternate Names

CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXCR4; CXC-R4; CXCR-4; D2S201E; D2S201ESDF-1 receptor; FB22; Fusin; HM89; HM89NPYRL; HSY3RR; LAP3; LAP-3; LCR1; LESTR; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3R; NPY3RWHIM; NPYR; NPYRL; NPYY3R; pCXCR4; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor; WHIMS

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Related Research Areas

Citations for Mouse CXCR4 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Integrins &alpha4&beta1 and &alphaV&beta3 are Reduced in Endothelial Progenitor Cells from Diabetic Dyslipidemic Mice and May Represent New Targets for Therapy in Aortic Valve Disease
Authors: A Filippi, A Constantin, N Alexandru, G Voicu, CA Constantin, D Rebleanu, M Fenyo, D Simionescu, A Simionescu, I Manduteanu, A Georgescu
Cell Transplant, 2020;29(0):9.6369E+14.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Infection of Female BWF1 Lupus Mice with Malaria Parasite Attenuates B Cell Autoreactivity by Modulating the CXCL12/CXCR4 Axis and Its Downstream Signals PI3K/AKT, NFkappaB and ERK.
Authors: Badr G, Sayed A, Abdel-Maksoud M, Mohamed A, El-Amir A, Abdel-Ghaffar F, Al-Quraishy S, Mahmoud M
PLoS ONE, 2015;10(4):e0125340.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Chemokine receptor CX3CR1 contributes to macrophage survival in tumor metastasis.
Authors: Zheng, Jiao, Yang, Min, Shao, Jianghua, Miao, Yanju, Han, Jiahuai, Du, Jie
Mol Cancer, 2013;12(1):141.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
G protein-coupled receptor kinase-3-deficient mice exhibit WHIM syndrome features and attenuated inflammatory responses.
Authors: Tarrant, Teresa K, Billard, Matthew, Timoshchenko, Roman G, McGinnis, Marcus W, Serafin, D Stephe, Foreman, Oded, Esserman, Denise A, Chao, Nelson J, Lento, William, Lee, David M, Patel, Dhavalku, Siderovski, David P
J Leukoc Biol, 2013;94(6):1243-51.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
MicroRNA126 contributes to granulocyte colony-stimulating factor-induced hematopoietic progenitor cell mobilization by reducing the expression of vascular cell adhesion molecule 1.
Authors: Salvucci O, Jiang K, Gasperini P
Haematologica, 2012;97(6):818-26.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
CXCL12 enhances exogenous CD4+CD25+ T cell migration and prevents embryo loss in non-obese diabetic mice.
Authors: Lin Y, Xu L, Jin H, Zhong Y, Di J, Lin QD
Fertil. Steril., 2009;91(6):2687-96.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
The angiogenic response is dictated by beta3 integrin on bone marrow-derived cells.
Authors: Feng W, McCabe NP, Mahabeleshwar GH, Somanath PR, Phillips DR, Byzova TV
J. Cell Biol., 2008;183(6):1145-57.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry