Mouse CXCR4 PerCP-conjugated Antibody

FAB21651C Datasheet / COA / SDS

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FAB21651C-025

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Detection of CXCR4 in Mouse Thymocytes by Flow Cytometry.

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Mouse CXCR4 PerCP-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CXCR4 transfectants. Does not stain irrelevant transfectants.

Source

Monoclonal Rat IgG_2B Clone # 247506

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Y3 rat myeloid cell line transfected with mouse CXCR4
Met1-Ser359
Accession # P70658

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of CXCR4 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse CXCR4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB21651C, filled histogram) or isotype control antibody (Catalog # IC013C, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR4

CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and-2 viral entry into cells.

Entrez Gene IDs

7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)

Alternate Names

CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXCR4; CXC-R4; CXCR-4; D2S201ESDF-1 receptor; FB22; Fusin; HM89NPYRL; HSY3RR; LAP3; LCR1; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3RWHIM; NPYR; NPYY3R; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor