Mouse Cystatin D/Cst10 Antibody

Catalog # Availability Size / Price Qty
AF6255
AF6255-SP

15% Off Limited-time Savings on Your Reagents, Assays, and Kits
Now through September 27th, you can save 15% on Bio-Techne reagents, assays, and kits (including Novus, Tocris, and R&D Systems). Use promotion code SAVE15 at checkout on orders over $1,000. See terms and conditions for offer details.

Detection of Mouse Cystatin D/Cst10 by Western Blot.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Mouse Cystatin D/Cst10 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Cystatin D/Cst10 in direct ELISAs and Western blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human (rh) Cst1, rhCst2, and rhCst3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cystatin D/Cst10
Asn30-Phe148
Accession # NP_067380
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse Cystatin D/Cst10 antibody by Western Blot. View Larger

Detection of Mouse Cystatin D/Cst10 by Western Blot. Western blot shows lysates of mouse salivary gland tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Cystatin D/Cst10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6255) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cystatin D/Cst10 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin D/Cst10

Cystatin D (Cystatin 10; also Carminerin) is a 17 kDa member of the type 2 cystatin family of proteins. It is expressed in salivary gland, skin, prehypertrophic and hypertrophic chondrocytes. Within the context of endochondral ossification, it may both induce chondrocyte apoptosis and initiate the process of cartilage calcification. Although Cystatin D contains a key cystatin motif (Gln-x-Val-x-Gly), Cystatin D does not appear to inhibit cysteine proteases. Mouse Cystatin D is 148 amino acids (aa) in length. It contains one cystatin-like domain (aa 39-145). There are two potential splice variants. One shows a deletion of aa 84-121, while a second shows a Val substitution for aa 122-148. Full-length mouse Cystatin D shares 78% aa identity with rat Cystatin D. There appears to be no direct human counterpart to mouse Cystatin D, as both human cystatin-C and -D share less that 40% aa identity with mouse Cystatin D.

Entrez Gene IDs
58214 (Mouse); 366219 (Rat)
Alternate Names
Cst10; Cystatin 10; cystatin D; Cystatin D/Cst10; DD72

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse Cystatin D/Cst10 Antibody

There are currently no reviews for this product. Be the first to review Mouse Cystatin D/Cst10 Antibody and earn rewards!

Have you used Mouse Cystatin D/Cst10 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review