Mouse Dectin-1/CLEC7A PerCP-conjugated Antibody

FAB17561C has been discontinued. View all Dectin-1/CLEC7A products.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Dectin‑1/CLEC7A in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Dectin-1 is observed and no cross-reactivity with recombinant mouse Dectin-2 or rhDLEC is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 218820
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Dectin‑1/CLEC7A
    Phe69-Leu244
    Accession # Q6QLQ4
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Dectin‑1/CLEC7A in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Dectin‑1/CLEC7A PerCP‑conjugated Monoclonal Antibody (Catalog # FAB17561C, filled histogram) or isotype control antibody (Catalog # IC006C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 43 kDa type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner (1, 2). Mature mouse Dectin-1 is a 244 amino acid (aa) glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain (3). The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin‑1, respectively. It shares 25%‑34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region (4). Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells (5). It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (7). Yeast beta -glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). It co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection (15, 16).

  • References:
    1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
    2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
    3. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:20157.
    4. Heinsbroek, S.E.M. et al. (2006) J. Immunol. 176:5513.
    5. Taylor, P.R. et al. (2002) J. Immunol. 169:3876. 
    6. Willment, J.A. et al. (2003) J. Immunol. 171:4569. 
    7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
    8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
    9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
    10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
    11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
    12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
    13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
    14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
    15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
    16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
  • Entrez Gene IDs:
    64581 (Human); 56644 (Mouse)
  • Alternate Names:
    Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1
Related Research Areas

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