Mouse EphB3 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse EphB3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant rat EphB1, recombinant mouse (rm) EphA4, rmEphB2, rmEphB4, and rmEphB6 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse EphB3
    Accession # P54754
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of EphB3 in COLO 205 Human Cell Line by Flow Cytometry. COLO 205 human colorectal adenocarcinoma cell line was stained with Goat Anti-Mouse EphB3 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB432A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: EphB3
EphB3, also known as Cek10, Tyro6, Sek4, Hek2, and Mdk5, is a 130 kDa member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference, but have a common structural organization. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1). The 525 amino acid (aa) extracellular domain (ECD) of mature mouse EphB3 contains a ligand binding domain followed by a cysteine rich region and two fibronectin type III domains. The 418 aa cytoplasmic domain contains a tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif (2). Within the ECD, mouse EphB3 shares 96% and 99% aa sequence identity with human and rat EphB3, respectively. Binding of EphB3 to its ligands Ephrin-B1, B2, and B3 triggers forward signaling through EphB3 as well as reverse signaling through the Ephrin (1, 3). EphB3 also interacts in cis with the receptor tyrosine kinase Ryk (4). Activation of its kinase is required for some but not all of the effects of EphB3 on cellular adhesion, motility, and morphology (5). EphB3 is widely expressed during development and in the adult, where it shows a complementary tissue distribution to the Ephrin-B ligands (6‑9). EphB3 function is important in vascular, nervous system, thymocyte, and palate development (6, 7, 10‑12). It directs embyronic neuronal axon pathfinding, and its upregulation on local macrophages following neuronal injury promotes the growth of regenerating axons (10, 13). EphB3 inhibits colorectal carcinogenesis and invasion by preventing the migration of tumor cells out of the intestinal crypt (9, 14). EphB3 function is supported by the cooperative action of EphB2 in several of these processes (6, 10‑12, 15).
  • References:
    1. Pasquale, E.B. (2008) Cell 133:38.
    2. Overman, R.C. et al. (2014) Protein Sci. 23:627.
    3. Pasquale, E.B (2004) Nat. Neurosci. 7:417.
    4. Trivier, E. and T.S. Ganesan (2002) J. Biol. Chem. 277:23037.
    5. Miao, H. et al. (2005) J. Biol. Chem. 280:923.
    6. Adams, R.H. et al. (1999) Genes Dev. 13:295.
    7. Krull, C.E. et al. (1997) Curr. Biol. 7:571.
    8. Willson, C.A. et al. (2006) J. Mol. Histol. 37:369.
    9. Cortina, C. et al. (2007) Nat. Genet. 39:1376.
    10. Birgbauer, E. et al. (2000) Development 127:1231.
    11. Alfaro, D. et al. (2008) Immunology 125:131.
    12. Risley, M. et al. (2009) Mech. Dev. 126:230.
    13. Liu, X. et al. (2006) J. Neurosci. 26:3087.
    14. Batlle, E. et al. (2005) Nature 435:1126.
    15. Holmberg, J. et al. (2006) Cell 125:1151.
  • Long Name:
    Eph Receptor B3
  • Entrez Gene IDs:
    2049 (Human); 13845 (Mouse)
  • Alternate Names:
    Cek10; EC 2.7.10; EC; EK2; EPH receptor B3; EphB3; EPH-like kinase 2; EPH-like tyrosine kinase-2; ephrin type-B receptor 3; ETK2Hek2; Hek2; human embryo kinase 2; Mdk5; Sek4; Tyro6; Tyrosine-protein kinase TYRO6
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Goat IgG APC-conjugated Antibody

Ctrl IC108A 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse EphB3 APC-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee


Customer Information