Mouse F4/80 Alexa Fluor® 594-conjugated Antibody

FAB5580T Datasheet / COA / SDS

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FAB5580T-100UG

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Mouse F4/80 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse F4/80/EMR1.

Source

Monoclonal Rat IgG2a Clone # 521204

Immunogen

NS0 mouse myeloma cell line transfected with mouse F4/80/EMR1
Gln28-Gly931
Accession # Q61549

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 594

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

RAW 264.7 mouse monocyte/macrophage cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: F4/80

F4/80, also known as EMR1, is a 160 kDa extensively glycosylated protein in the EGF-TM7 family of adhesion molecules. It is primarily expressed on macrophages and dendritic cells in mouse but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance.

Entrez Gene IDs

13733 (Mouse); 316137 (Rat)

Alternate Names

ADGRE1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; Mouse EMR1; Rat EMR1; TM7LN3