Detects mouse Fas Ligand/TNFSF6 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) TRAIL and recombinant mouse (rm) TRANCE is observed and 5% cross-reactivity with rhFas Ligand, recombinant rat Fas Ligand, rmTNF-alpha, rhAPRIL, and rhVEGI is observed and no cross-reactivity with rhGITR Ligand and rhLIGHT is observed.
Monoclonal Rat IgG1 Clone # 101624
Protein A or G purified from hybridoma culture supernatant
Detection of Fas Ligand/TNFSF6 in HEK293 Human Cell Line Transfected with Mouse Fas Ligand/TNFSF6 by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with mouse Fas Ligand/TNFSF6 was stained with Rat Anti-Mouse Fas Ligand/TNFSF6 APC‑conjugated Monoclonal Antibody (Catalog # FAB526A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Fas Ligand/TNFSF6
Fas Ligand (FasL) is a 40 kDa type II transmembrane protein belonging to the TNF super family. In the new TNF super family nomenclature, FasL is referred to as TNFSF6. The specific receptor for FasL is Fas (CD95, Apo-1), a 45 kDa type I transmembrane protein that is a member of the TNF receptor family. FasL is predominantly expressed on activated T cells and NK cells, while Fas is expressed on various types of cells. The Fas/FasL system plays a crucial role in modulating immune response by inducing cell apoptosis to maintain homeostasis, self-tolerance of lymphocytes, and immune privilege. FasL was reported to be a potent chemoattractant for neutrophils, suggesting a novel proinflammatory function of this molecule. Like other members of the TNF family, the membrane-bound FasL can be cleaved by metalloproteinase to generate a soluble Fas ligand (sFasL) which is mainly a non-covalently linked homotrimer. It has been shown that membrane-bound TNF‑ alpha and FasL are primary activators of their receptors. In contrast to soluble TNF‑ alpha which has potent cytotoxicity, sFasL is much less cytotoxic. Studies have shown that sFasL may competitively inhibit the killing effect of membrane FasL indicating that the cleaving of membrane FasL might be a mechanism to down‑regulate its activity. Over amino acids (aa) # 132-279, mouse FasL shares 83% aa sequence identity with human FasL.
Suda, T. et al. (1993) Cell 75:1169.
Kägi, D. et al. (1994) Science 265:528.
Schneider, P. et al. (1998) J. Exp. Med. 187:1205.
Have you used Mouse Fas Ligand/TNFSF6 APC-conjugated Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.