Mouse Fas Ligand/TNFSF6 APC-conjugated Antibody

FAB526A has been discontinued. View all Fas Ligand/TNFSF6 products.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Fas Ligand/TNFSF6 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) TRAIL and recombinant mouse (rm) TRANCE is observed and 5% cross-reactivity with rhFas Ligand, recombinant rat Fas Ligand, rmTNF-alpha, rhAPRIL, and rhVEGI is observed and no cross-reactivity with rhGITR Ligand and rhLIGHT is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 101624
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Fas Ligand/TNFSF6
    Pro132-Leu279
    Accession # P41047
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Fas Ligand/TNFSF6 in HEK293 Human Cell Line Transfected with Mouse Fas Ligand/TNFSF6 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse Fas Ligand/TNFSF6 was stained with Rat Anti-Mouse Fas Ligand/TNFSF6 APC‑conjugated Monoclonal Antibody (Catalog # FAB526A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Fas Ligand/TNFSF6

Fas Ligand (FasL) is a 40 kDa type II transmembrane protein belonging to the TNF super family. In the new TNF super family nomenclature, FasL is referred to as TNFSF6. The specific receptor for FasL is Fas (CD95, Apo-1), a 45 kDa type I transmembrane protein that is a member of the TNF receptor family. FasL is predominantly expressed on activated T cells and NK cells, while Fas is expressed on various types of cells. The Fas/FasL system plays a crucial role in modulating immune response by inducing cell apoptosis to maintain homeostasis, self-tolerance of lymphocytes, and immune privilege. FasL was reported to be a potent chemoattractant for neutrophils, suggesting a novel proinflammatory function of this molecule. Like other members of the TNF family, the membrane-bound FasL can be cleaved by metalloproteinase to generate a soluble Fas ligand (sFasL) which is mainly a non-covalently linked homotrimer. It has been shown that membrane-bound TNF‑ alpha and FasL are primary activators of their receptors. In contrast to soluble TNF‑ alpha which has potent cytotoxicity, sFasL is much less cytotoxic. Studies have shown that sFasL may competitively inhibit the killing effect of membrane FasL indicating that the cleaving of membrane FasL might be a mechanism to down‑regulate its activity. Over amino acids (aa) # 132-279, mouse FasL shares 83% aa sequence identity with human FasL.

  • References:
    1. Suda, T. et al. (1993) Cell 75:1169.
    2. Kägi, D. et al. (1994) Science 265:528.
    3. Schneider, P. et al. (1998) J. Exp. Med. 187:1205.
    4. Seino, K. et al. (1998) J. Immunol. 161:4484.
  • Entrez Gene IDs:
    356 (Human); 14103 (Mouse); 25385 (Rat)
  • Alternate Names:
    apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1CD95L; APTL; CD178 antigen; CD178; CD95L; CD95-L; Fas antigen ligand; Fas ligand (TNF superfamily, member 6); Fas Ligand; FASLCD95 ligand; FASLG; TNFSF6; TNFSF6FasL; tumor necrosis factor (ligand) superfamily, member 6; tumor necrosis factor ligand superfamily member 6
Related Research Areas

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