Mouse Gr-1/Ly-6G Alexa Fluor® 700-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Gr‑1/Ly‑6G in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse Integrin aM/CD11b APC-conjugated Monoclonal Antibody (Catalog # FAB1124A) and either (A) Rat Anti-Mouse Gr-1/Ly-6G Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB1037N) or (B) Rat IgG2BAlexa Fluor 700 Isotype Control (Catalog # IC013N). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Ly-6G/Ly-6C (Gr-1)
The myeloid differentiation antigen Gr-1 is a member of the Ly-6 family, Ly-6G (1). The RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). In the periphery, this antibody specifically recognized granulocytes (3, 4).
- Spangrude, G.J. et al. (1988) Science 241:58.
- Fleming, T.J. et al. (1993) J. Immunol. 151:2399.
- Lewinsohn, D.M. et al.(1987) J. Immunol. 147:22.
- Lagasse, E. and I.L. Weissman (1996) J. Immunol. Methods 197:139.
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