Mouse IFN-gamma Antibody

Catalog # Availability Size / Price Qty
MAB485-SP
MAB485-100
MAB485-500
Mouse IFN-gamma  Antibody in Data
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Product Details
Citations (18)
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Mouse IFN-gamma Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IFN-gamma in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) IFN‑ gamma, rrIFN‑ gamma, rpIFN‑ gamma, rrmIFN‑ gamma, rfeIFN‑ gamma, or rcaIFN‑ gamma.
Source
Monoclonal Rat IgG2A Clone # 37895
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IFN-gamma
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IFN-gamma (Catalog # 485-MI)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below
Neutralization
Measured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND50) is typically 0.075-0.3 µg/mL in the presence of 2.5 ng/mL Recombinant Mouse IFN‑ gamma.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection of IFN-? antibody in Mouse Splenoctyes antibody by Flow Cytometry. View Larger

Detection of IFN‑ gamma in Mouse Splenoctyes by Flow Cytometry. Mouse splenocytes either (A) stimulated to induce Th1 cells or (B) unstimulated were stained with Rat Anti-Mouse IFN-gamma Monoclonal Antibody (Catalog # MAB485) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) and Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A). Quadrant markers were set based on control antibody staining (Catalog # MAB006). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Neutralization IFN‑ gamma  Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ gamma  Antibody. View Larger

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ gamma Antibody. Recombinant Mouse IFN-gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IFN-gamma Monoclonal Antibody (Catalog # MAB485). The ND50 is typically 0.075-0.3 µg/mL.

Intracellular Staining by Flow Cytometry View Larger

Detection of IFN‑ gamma in Mouse Splenoctyes by Flow Cytometry. Mouse splenocytes either (A) stimulated for 4 hours with Cell Activation Cocktail 500x (5476) or (B) unstimulated were stained with Rat Anti-Mouse IFN-gamma Monoclonal Antibody (Catalog # MAB485) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B) and Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (FAB554A). Quadrant markers were set based on control antibody staining (MAB006). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma

Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. It shares 86% amino acid (aa) sequence identity with rat IFN-gamma, 38-44% with bovine, canine, cotton rat, equine, feline, human, porcine and rhesus IFN‑ gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

Long Name
Interferon gamma
Entrez Gene IDs
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

Product Datasheets

Citations for Mouse IFN-gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Ablation of RhoA impairs Th17 cell differentiation and alleviates house dust mite-triggered allergic airway inflammation
    Authors: JQ Yang, KW Kalim, Y Li, Y Zheng, F Guo
    J. Leukoc. Biol., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Intraperitoneal infusion of mesenchymal stem cell attenuates severity of collagen antibody induced arthritis
    Authors: Y Nam, SM Jung, YA Rim, H Jung, K Lee, N Park, J Kim, Y Jang, YB Park, SH Park, JH Ju
    PLoS ONE, 2018;13(6):e0198740.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  3. Crucial role of OX40/OX40L signaling in a murine model of asthma
    Authors: W Lei, D Zeng, G Liu, Y Zhu, J Wang, H Wu, J Jiang, J Huang
    Mol Med Rep, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  4. Armc5 deletion causes developmental defects and compromises T-cell immune responses
    Authors: Y Hu, L Lao, J Mao, W Jin, H Luo, T Charpentie, S Qi, J Peng, B Hu, MM Marcinkiew, A Lamarre, J Wu
    Nat Commun, 2017;8(0):13834.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  5. CD4(+) T cells from food allergy model are resistant to TCR-dependent apoptotic induction.
    Authors: He W, Yang C, Xia L, Zhao M, Ge R, Huang H, Ji Q, Yang P, Liu Z
    Cytokine, 2014;68(1):32-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Transcriptional regulator CTR9 inhibits Th17 differentiation via repression of IL-17 expression.
    Authors: Yoo, Hyeon-Se, Choi, Yongwook, Ahn, Narae, Lee, Saseong, Kim, Wan-Uk, Jang, Min Seon, Jang, Myoung H, Kim, Yon Su, Yoo, Joo-Yeon
    J Immunol, 2014;192(4):1440-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  7. The effect of conditional EFNB1 deletion in the T cell compartment on T cell development and function.
    Authors: Jin W, Qi S, Luo H
    BMC Immunol., 2011;12(0):68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  8. IL-21 Promotes Skin Recruitment of CD4+ Cells and Drives IFN-{gamma}-Dependent Epidermal Hyperplasia.
    Authors: Sarra M, Caruso R, Cupi ML, Monteleone I, Stolfi C, Campione E, Diluvio L, Mazzotta A, Botti E, Chimenti S, Costanzo A, MacDonald TT, Pallone F, Monteleone G
    J. Immunol., 2011;186(9):5435-42.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  9. Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
    Authors: Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA
    FASEB J., 2011;25(1):409-19.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  10. IL-13 from Th2-type cells suppresses induction of antigen-specific Th1 immunity in a T-cell lymphoma.
    Authors: Deepak P, Kumar S, Kishore D, Acharya A
    Int. Immunol., 2010;22(1):53-63.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  11. Chlamydial respiratory infection during allergen sensitization drives neutrophilic allergic airways disease.
    Authors: Horvat JC, Starkey MR, Kim RY, Beagley KW, Preston JA, Gibson PG, Foster PS, Hansbro PM
    J. Immunol., 2010;184(8):4159-69.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  12. Controlled delivery of T-box21 small interfering RNA ameliorates autoimmune alopecia (Alopecia Areata) in a C3H/HeJ mouse model.
    Authors: Nakamura M, Jo J, Tabata Y, Ishikawa O
    Am. J. Pathol., 2008;172(3):650-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  13. Crucial role of the interleukin-6/interleukin-17 cytokine axis in the induction of arthritis by glucose-6-phosphate isomerase.
    Authors: Iwanami K, Matsumoto I, Tanaka-Watanabe Y, Inoue A, Mihara M, Ohsugi Y, Mamura M, Goto D, Ito S, Tsutsumi A, Kishimoto T, Sumida T
    Arthritis Rheum., 2008;58(3):754-63.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  14. Mitochondrial structural changes and dysfunction are associated with experimental allergic asthma.
    Authors: Mabalirajan U, Dinda AK, Kumar S, Roshan R, Gupta P, Sharma SK, Ghosh B
    J. Immunol., 2008;181(5):3540-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  15. TH17 cells mediate steroid-resistant airway inflammation and airway hyperresponsiveness in mice.
    Authors: McKinley L, Alcorn JF, Peterson A, Dupont RB, Kapadia S, Logar A, Henry A, Irvin CG, Piganelli JD, Ray A, Kolls JK
    J. Immunol., 2008;181(6):4089-97.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Maternal immune activation alters fetal brain development through interleukin-6.
    Authors: Smith SE, Li J, Garbett K, Mirnics K, Patterson PH
    J. Neurosci., 2007;27(40):10695-702.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  17. A new RXR agonist, HX630, suppresses intimal hyperplasia in a mouse blood flow cessation model.
    Authors: Haraguchi G, Suzuki J, Kosuge H, Ogawa M, Koga N, Muto S, Itai A, Kagechika H, Shudo K, Isobe M
    J. Mol. Cell. Cardiol., 2006;41(5):885-92.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Dendritic cells and NK cells stimulate bystander T cell activation in response to TLR agonists through secretion of IFN-alpha beta and IFN-gamma.
    Authors: Kamath AT, Sheasby CE, Tough DF
    J. Immunol., 2005;174(2):767-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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