Mouse IGF-I Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IGF-I in direct ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) IGF‑I, rmIGF-II, rmIGFBP-2, rmIGFBP-5, and rmIGFBP-6.
  • Source
    Monoclonal Hamster IgG Clone # 126002
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse IGF-I
    Gly49-Ala118
    Accession # P05017
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse IGF-I (Catalog # 791-MG)
    • Mouse IGF-I Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse IGF‑I Antibody (Catalog # MAB791)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse IGF‑I Biotinylated Antibody (Catalog # BAF791)
  • ELISA Standard
     
    Recombinant Mouse IGF-I Protein, CF (Catalog # 791-MG)
  • Neutralization
    Measured by its ability to neutralize IGF‑I-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 15 ng/mL Recombinant Mouse IGF‑I.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IGF‑I and Neutralization by Mouse IGF‑I Antibody. Recombinant Mouse IGF‑I (Catalog # 791-MG) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF‑I (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IGF-I Monoclonal Antibody (Catalog # MAB791). The ND50 is typically 0.3-1.5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGF-I

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Mouse IGF-I is synthesized as two precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature mouse IGF-I shares 94% and 99% amino acid (aa) sequence identity with human and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse IGF-II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects (1). IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4-7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).

  • References:
    1. Philippou, A. et al. (2007) In Vivo 21:45.
    2. Bell, G.I. et al. (1986) Nucleic Acids Res. 14:7873.
    3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
    4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
    5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
    6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
    7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
    8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
    9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
    10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.
  • Long Name:
    Insulin-like Growth Factor I
  • Entrez Gene IDs:
    3479 (Human); 16000 (Mouse); 24482 (Rat)
  • Alternate Names:
    IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Insulin-like growth factor 1 is a direct HOXA9 target important for hematopoietic transformation.
    Authors: Steger J, Fuller E, Garcia-Cuellar M, Hetzner K, Slany R
    Leukemia, 2015;29(4):901-8.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  2. Spatial distribution of insulin-like growth factor binding protein-2 following hypoxic-ischemic injury.
    Authors: Fletcher L, Isgor E, Sprague S, Williams L, Alajajian B, Jimenez D, Digicaylioglu M
    BMC Neurosci, 2013;14(0):158.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Capture
  3. IGFBP-3 is a metastasis suppression gene in prostate cancer.
    Authors: Mehta HH, Gao Q, Galet C
    Cancer Res., 2011;71(15):5154-63.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Development
  4. Evidence of a role for insulin-like growth factor binding protein (IGFBP)-3 in metabolic regulation.
    Authors: Yamada PM, Mehta HH, Hwang D
    Endocrinology, 2010;151(12):5741-50.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Development
  5. Dietary feeding of silibinin inhibits prostate tumor growth and progression in transgenic adenocarcinoma of the mouse prostate model.
    Authors: Raina K, Blouin MJ, Singh RP, Majeed N, Deep G, Varghese L, Glode LM, Greenberg NM, Hwang D, Cohen P, Pollak MN, Agarwal R
    Cancer Res., 2007;67(22):11083-91.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
Secondary Antibodies
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Mouse Anti-Hamster IgG Antibody

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Mouse Anti-Hamster IgG APC-conjugated Antibody

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Mouse Anti-Hamster IgG Biotinylated Antibody

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Mouse Anti-Hamster IgG PE-conjugated Antibody

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