Mouse IGF-I Antibody

(10 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IGF‑I in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human IGF-I is observed, and less than 2% cross-reactivity with recombinant mouse IGF-II is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse IGF-I
    Gl33-Ala102
    Accession # Q8CAR0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse IGF-I (Catalog # 791-MG)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize IGF‑I-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K. P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 0.2-1.0 µg/mL in the presence of 15 ng/mL Recombinant Mouse IGF‑I.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IGF‑I and Neutralization by Mouse IGF‑I Antibody. Recombinant Mouse IGF‑I (Catalog # 791-MG) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF‑I (15 ng/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-Mouse IGF-I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791). The ND50 is typically 0.2-1.0 µg/mL.
Immunohistochemistry
IGF‑I in Mouse Embryo. IGF‑I was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse IGF‑I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing brain and muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGF-I

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Mouse IGF-I is synthesized as two precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature mouse IGF-I shares 94% and 99% aa sequence identity with human and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse IGF‑II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects (1). IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4-7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).

  • References:
    1. Philippou, A. et al. (2007) In Vivo 21:45.
    2. Bell, G.I. et al. (1986) Nucleic Acids Res. 14:7873.
    3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
    4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
    5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
    6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
    7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
    8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
    9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
    10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.
  • Long Name:
    Insulin-like Growth Factor I
  • Entrez Gene IDs:
    3479 (Human); 16000 (Mouse); 24482 (Rat)
  • Alternate Names:
    IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Combined Effects of Androgen and Growth Hormone on Osteoblast Marker Expression in Mouse C2C12 and MC3T3-E1 Cells Induced by Bone Morphogenetic Protein
    Authors: K Kimura, T Terasaka, N Iwata, T Katsuyama, M Komatsubar, R Nagao, K Inagaki, F Otsuka
    J Clin Med, 2017;6(1):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  2. Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism
    Authors: C Zhao, W Zhang, Y Zhao, Y Yang, H Luo, G Ji, E Dong, H Deng, S Lin, Y Wei, H Yang
    Sci Rep, 2016;6(0):19404.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  3. Survival of neural stem cell grafts in the lesioned spinal cord is enhanced by a combination of treadmill locomotor training via insulin-like growth factor-1 signaling.
    Authors: Hwang D, Shin H, Kwon M, Choi J, Ryu B, Kim B
    J Neurosci, 2014;34(38):12788-800.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  4. Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength.
    Authors: Brisson, Becky K, Spinazzola, Janelle, Park, SooHyun, Barton, Elisabet
    Am J Physiol Endocrinol Metab, 2014;306(8):E965-74.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  5. Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9.
    Authors: Koronyo-Hamaoui M, Ko MK, Koronyo Y, Azoulay D, Seksenyan A, Kunis G, Pham M, Bakhsheshian J, Rogeri P, Black KL, Farkas DL, Schwartz M
    J. Neurochem., 2009;111(6):1409-24.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  6. Role of IGF signaling in olfactory sensory map formation and axon guidance.
    Authors: Scolnick JA, Cui K, Duggan CD, Xuan S, Yuan XB, Efstratiadis A, Ngai J
    Neuron, 2008;57(6):847-57.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Low-dose GH supplementation reduces the TLR2 and TNF-alpha expressions in visceral fat.
    Authors: Kubota Y, Unoki H, Bujo H, Rikihisa N, Udagawa A, Yoshimoto S, Ichinose M, Saito Y
    Biochem. Biophys. Res. Commun., 2008;368(1):81-7.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  8. Induction and blockage of oligodendrogenesis by differently activated microglia in an animal model of multiple sclerosis.
    Authors: Butovsky O, Landa G, Kunis G, Ziv Y, Avidan H, Greenberg N, Schwartz A, Smirnov I, Pollack A, Jung S, Schwartz M
    J. Clin. Invest., 2006;116(4):905-15.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  9. Glatiramer acetate fights against Alzheimer's disease by inducing dendritic-like microglia expressing insulin-like growth factor 1.
    Authors: Butovsky O, Koronyo-Hamaoui M, Kunis G, Ophir E, Landa G, Cohen H, Schwartz M
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(31):11784-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  10. Microglia activated by IL-4 or IFN-gamma differentially induce neurogenesis and oligodendrogenesis from adult stem/progenitor cells.
    Authors: Butovsky O, Ziv Y, Schwartz A, Landa G, Talpalar AE, Pluchino S, Martino G, Schwartz M
    Mol. Cell. Neurosci., 2006;31(1):149-60.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
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