Detection of Mouse IgG2B Primary Antibodies by Western Blot.
Western blot shows lysates of HepG2 and Jurkat cell lines. PVDF membrane was probed with anti-HSP90 (Catalog # MAB3286, Mouse IgG2B isotype), anti-HSP60 (Catalog # MAB1800, Mouse IgG2B isotype), anti-GAPDH (Catalog # MAB5718,Mouse IgG1 isotype), anti-Peroxiredoxin 6 (Catalog # MAB3490, Mouse IgG3 isotype), and anti-SOD1 (Catalog # MAB3418, Mouse IgG2A isotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG2B (Catalog # MAB9636) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for IgG2B isotype antibodies at approximately 60 and 90 kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018) but not Rabbit Anti-Mouse IgG2B Monoclonal Antibody (Catalog # MAB9636). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Immunoglobulin G2B Fc
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