Detects mouse IGSF8/CD316 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human IGSF8 is observed and less than 1% cross-reactivity with recombinant mouse IGSF4 is observed.
Detection of IGSF8/CD316 in Neuro‑2A Mouse Cell Line by Flow Cytometry. Neuro‑2A mouse neuroblastoma cell line was stained with Goat Anti-Mouse IGSF8/CD316 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3117, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
IGSF8/CD316 in Neuro‑2A Mouse Cell Line. IGSF8/CD316 was detected in immersion fixed Neuro‑2A mouse neuroblastoma cell line using Goat Anti-Mouse IGSF8/CD316 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3117) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
IGSF8, also known as PGRL (PG Regulatory-Like Protein), KASP (KAI/CD82 Associated Protein) and EWI-2 (Glu-Trp-Ile motif 2), is a widely expressed transmembrane adhesion protein. It interacts with beta 1 Integrins and various tetraspanins including CD9, CD81 and CD82. IGSF8 contains four extracellular Ig-like domains. IGSF8 overexpression in transformed cells inhibits cell migration and suppresses cancer metastatic potential. Mouse and human IGSF8 share 90% amino acid sequence identity.
Immunoglobulin Superfamily, Member 8
Entrez Gene IDs:
CD316 antigen; CD316; CD81P3; CD81P3LIR-D1; EWI2; EWI-2; EWI2CD81 partner 3; Glu-Trp-Ile EWI motif-containing protein 2; IGSF8; immunoglobulin superfamily, member 8; KCT4; KCT-4; Keratinocytes-associated transmembrane protein 4; PGRL; PGRLimmunoglobulin superfamily member 8
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