Mouse IL-15 R alpha APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse IL-15 R alpha in direct ELISAs.
  • Source
    Monoclonal Rat IgG2B Clone # 888220
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IL-15 R alpha
    Accession # Q60819
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of IL‑15 R alpha in C57BL/6 Mouse Bone Marrow Cells by Flow Cytometry. C57BL/6 mouse bone marrow cells treated with 20 ng/mL Recombinant Mouse GM‑CSF (Catalog # 415-ML) for 5 days, 1ng/mL LPS on day 6, and harvested on day 7 were stained with Rat Anti-Mouse IL‑15 R alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB5511A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-15 R alpha
Interleukin 15 Receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta and IL-2 R gamma c, through the heterodimeric complex of IL-2 receptors beta and gamma common, and through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF‑ alpha ‑mediated apoptosis in fibroblasts by competing with TNFRI for TRAF2 binding.
  • Long Name:
    Interleukin 15 Receptor alpha
  • Entrez Gene IDs:
    3601 (Human); 16169 (Mouse)
  • Alternate Names:
    CD215 antigen; CD215; IL-15 receptor subunit alpha; IL15R alpha; IL15RA; IL-15Ra; IL-15R-alpha; interleukin 15 receptor, alpha; interleukin-15 receptor subunit alpha; MGC104179
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B APC‑conjugated Isotype Control

Ctrl IC013A 3  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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