Mouse IL-17 RA/IL-17 R PE-conjugated Antibody

FAB448P has been discontinued. View all IL-17 RA/IL-17 R products.
(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-17 R in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human IL‑17 R is observed and less than 1% cross-reactivity with recombinant mouse (rm) IL-17 RC and rmIL-17 RD is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-17 R
    Extracellular domain
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑17 R in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse IL‑17 R PE-conjugated Polyclonal Antibody (Catalog # FAB448P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-17 RA/IL-17 R
IL-17 R, also known as IL-17 RA, is a 120 kDa type I transmembrane glycoprotein protein that plays a central role in inflammatory responses (1-3). Mature mouse IL‑17 R consists of a 291 amino acid (aa) extracellular domain, a 21 aa transmembrane segment, and a 521 aa cytoplasmic domain (4). The cytoplasmic domain contains a region homologous to the TIR domain of the TLR/IL-1 R family (5). Mouse IL-17 R shares 84% and 72% aa sequence identity with rat and human IL-17 R, respectively. Within the extracellular domain, it shares 18-25% sequence identity with mouse IL-17 RB, C, D, and E. While the expression of IL-17 is restricted to activated T cells, IL-17 R exhibits a broad tissue distribution (4). Even in the absence of ligand, IL-17 R exists on the cell surface as a multimer (6). IL-17 R can bind IL-17 but must associate with IL-17 RC to transduce signals (7, 8). Interestingly, human IL-17 R does not appear to form productive complexes with mouse IL-17 RC (8). The IL-17 R can also signal in response to IL-17F (9). IL-17 R ligation promotes T cell activation and the production of IL-6, G-CSF, SCF, and multiple pro-inflammatory chemokines (4, 7, 9, 10). IL-17A and IL-17F synergize with TNF-alpha in the induction of CXCL1, G-CSF, and IL-6 (9, 11). This effect requires the presence of both TNF RI and TNF RII (9). IL-17 interactions with IL-17 R also inhibit the TNF-alpha induced upregulation of fibroblast CCL5 and VCAM-1 (11). CCL5 and VCAM-1 induced effects are differentially sensitive to blockade with IL-17 R specific antibodies, suggesting that IL-17 R triggers divergent intracellular signals (11). In vivo, IL‑17 R activity is important for increased generation of neutrophils and their recruitment to sites of inflammation (10, 12, 13). IL-17 R is required for host defense against microbial infection and for the progression of arthritis from inflammation to destructive joint erosion (10, 13).
  • References:
    1. Iwakura, Y. and H. Ishigame (2006) J. Clin. Invest. 116:1218.
    2. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
    3. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
    4. Yao, Z. et al. (1995) Immunity 3:811.
    5. Novatchkova, M. et al. (2003) Trends Biochem. Sci. 28:226.
    6. Kramer, J.M. et al. (2006) J. Immunol. 176:711.
    7. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
    8. Toy, D. et al. (2006) J. Immunol. 177:36.
    9. McAllister, F. et al. (2005) J. Immunol. 175:404.
    10. Ye, P. et al. (2001) J. Exp. Med. 194:519.
    11. Schnyder, B. et al. (2005) Cytokine 31:191.
    12. Tan, W. et al. (2006) J. Immunol. 176:6186.
    13. Lubberts, E. et al. (2005) J. Immunol. 175:3360.
  • Long Name:
    Interleukin 17 Receptor
  • Entrez Gene IDs:
    23765 (Human); 16172 (Mouse); 312679 (Rat)
  • Alternate Names:
    CD217 antigen; CD217; Cdw217; CDw217interleukin 17 receptor; hIL-17R; IL-17 R; IL-17 RA; IL-17 receptor A; IL17RA; IL-17RA; IL-17RAMGC10262; IL17Rinterleukin-17 receptor A; interleukin 17 receptor A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Macrophages participate in IL-17-mediated inflammation.
    Authors: Barin JG, Baldeviano GC, Talor MV, Wu L, Ong S, Quader F, Chen P, Zheng D, Caturegli P, Rose NR, Cihakova D
    Eur. J. Immunol., 2012;42(3):726-36.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow

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