Detects mouse IL-1 beta /IL-1F2 in direct ELISAs.
In direct ELISAs, approximately 75%
cross-reactivity with recombinant guinea pig IL-1 beta, 5-15%
cross-reactivitiy with recombinant canine, cotton rat, feline, equine, and rat
IL-1 beta, and no cross-reactivity with recombinant human,
porcine, and rhesus IL-1 beta is observed. No cross-reactivity with recombinant
human (rh) Pro-IL-1 beta, rhIL-1F7, rhIL-1F10, rhIL-36 gamma, recombinant mouse (rm) IL-1 alpha, rmIL-18,
rmIL-36Ra, rmIL-36 alpha, or rmIL-36 beta is observed.
Monoclonal Rat IgG2B Clone # 166931
Protein A or G purified from hybridoma culture supernatant
Detection of IL‑1 beta /IL‑1F2 in LPS-treated Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were unstimulated (open histogram), or treated with 1 ug/mL LPS for 48 hours (filled histogram) and stained with Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB4013) or isotype control antibody (Catalog # MAB006, not shown), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113). Histograms are gated on blasting splenocytes. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 beta is a pro-inflammatory cytokine belonging to the IL-1 superfamily, designated IL-1F2. IL-1 beta and IL-1 alpha /IL-1F1 bind the same receptors but vary in expression and affinity. They share only 25% amino acid (aa) sequence identity and are produced from separate genes. IL-1 beta is synthesized as a 31 kDa pro-form that is cleaved intracellularly by caspase-1 to produce the mature active 17.5 kDa IL-1 beta. Mature mouse IL-1 beta shares 74% and 91% aa identity with human and rat IL-1 beta, respectively.
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