Mouse IL-1 beta /IL-1F2 Antibody

MAB4013 has been discontinued. View all IL-1 beta/IL-1F2 products.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-1 beta /IL-1F2 in direct ELISAs. In direct ELISAs, approximately 75% cross-reactivity with recombinant guinea pig IL-1 beta, 5-15% cross-reactivitiy with recombinant canine, cotton rat, feline, equine, and rat IL-1 beta, and no cross-reactivity with recombinant human, porcine, and rhesus IL-1 beta is observed. No cross-reactivity with recombinant human (rh) Pro-IL-1 beta, rhIL-1F7, rhIL-1F10, rhIL-36 gamma, recombinant mouse (rm) IL-1 alpha, rmIL-18, rmIL-36Ra, rmIL-36 alpha, or rmIL-36 beta is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 166931
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E.coli-derived recombinant mouse IL-1 beta /IL-1F2
    Val118-Ser269
    Accession # P10749
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑1 beta /IL‑1F2 in LPS-treated Mouse Splenocytes by Flow Cytometry. Mouse splenocytes  were unstimulated (open histogram), or treated with 1 ug/mL LPS for 48 hours (filled histogram) and stained with Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB4013) or isotype control antibody (Catalog # MAB006, not shown), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113). Histograms are gated on blasting splenocytes. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2

IL-1 beta is a pro-inflammatory cytokine belonging to the IL-1 superfamily, designated IL-1F2. IL-1 beta and IL-1 alpha /IL-1F1 bind the same receptors but vary in expression and affinity. They share only 25% amino acid (aa) sequence identity and are produced from separate genes. IL-1 beta is synthesized as a 31 kDa pro-form that is cleaved intracellularly by caspase-1 to produce the mature active 17.5 kDa IL-1 beta. Mature mouse IL-1 beta shares 74% and 91% aa identity with human and rat IL-1 beta, respectively.

 

  • Long Name:
    Interleukin 1 beta
  • Entrez Gene IDs:
    3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
  • Alternate Names:
    catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
Related Research Areas

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