Intracellular Staining by Flow Cytometry
|Detection of IL‑1 beta /IL‑1F2 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either untreated (open histogram) or treated with LPS for 48 hours (filled histogram) were stained with Rat Anti-Mouse IL‑1 beta /IL‑1F2 PerCP‑conjugated Monoclonal Antibody (Catalog # IC4013C) or isotype control antibody (Catalog # IC013C, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
IL-1 beta is a pro-inflammatory cytokine belonging to the IL-1 superfamily, designated IL-1F2. IL-1 beta and IL-1 alpha /IL-1F1 bind the same receptors but vary in expression and affinity. They share only 25% amino acid (aa) sequence identity and are produced from separate genes. IL-1 beta is synthesized as a 31 kDa pro-form that is cleaved intracellularly by caspase-1 to produce the mature active 17.5 kDa IL-1 beta. Mature mouse IL-1 beta shares 74% and 91% aa identity with human and rat IL-1 beta, respectively.
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