Mouse IL-21 PerCP-conjugated Antibody

IC594C has been discontinued. View all IL-21 products.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-21 in Western blots. Does not cross-react with recombinant human IL‑21, recombinant mouse (rm) IL‑15, or rmIL‑12.
  • Source
    Monoclonal Rat IgG2B Clone # 149204
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse IL‑21
    Pro25-Ser146
    Accession # Q9ES17
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry

Detection of IL‑21 in Th17-stimulated Mouse Splenocytes by Flow Cytometry. Th17-stimulated mouse splenocytes were stained with Rat Anti-Mouse IL‑21 PerCP‑conjugated Monoclonal Antibody (Catalog # IC594C, filled histogram) or isotype control antibody (Catalog # IC013C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). Histograms were gated on CD4splenocytes. View our protocol for Staining Intracellular Molecules.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-21

Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (Novel Interleukin Receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% aa identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gamma c) chain appears to play a role in IL-21 R signaling. The IL-21/IL-21 R interaction appear to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation.

  • References:
    1. Parrish-Novak, J. et al. (2000) Nature 408:57.
    2. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
    3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
    4. Asao, H. et al. (2001) J. Immunol. 167:1.
  • Long Name:
    Interleukin 21
  • Entrez Gene IDs:
    59067 (Human); 60505 (Mouse); 365769 (Rat); 442935 (Canine); 100344172 (Rabbit)
  • Alternate Names:
    IL21; IL-21; IL-21Za11interleukin-21; interleukin 21; interleukin-21 isoform
Related Research Areas

Have you used Mouse IL-21 PerCP-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information