Mouse IL-21 R PE-conjugated Antibody

(2 citations)   
  • Species Reactivity
  • Specificity
    Detects mouse IL-21 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human IL-21 R or recombinant mouse IL-2 R beta is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 155516
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-21 R
    Accession # Q9JHX3
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑21 R in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse IL‑21 R PE‑conjugated Monoclonal Antibody (Catalog # FAB5961P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-21 R

IL-21 R (interleukin-21 receptor) is a type I transmembrane glycoprotein within the class I cytokine receptor family, type 4 subfamily (1-5). Complex formation between IL-21 R and the common gamma  chain ( gamma c), also used for IL-2, IL-4, IL-7, IL-9, IL-13 and IL-15 receptors, is required for signaling (6, 7). Mouse IL-21 R cDNA encodes 521 amino acid (aa) including a 19 aa signal peptide, a 218 aa extracellular domain (ECD) with 4 conserved cysteine residues, a fibronectin type III domain, and a WSXWS motif, a 21 aa transmembrane domain and a 271 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation (4, 5). One such site, pY510, mediates STAT binding (1, 2). The mouse IL-21 R ECD shares 69%, 91%, 65%, 63% and 58% aa identity with human, rat, equine, canine and bovine IL-21 R, respectively. One potential 447 aa isoform, with an alternate start site at aa 83, lacks the four conserved ECD cysteines. IL‑21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes (1, 3-5). Both IL-21 and IL-4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (8). B cell IL-21 R engagement induces BLIMP-1 (which mediates plasma cell differentiation), and is important for memory responses (1, 9, 10).  IL-21 R engagement on mouse NK cells enhances their cytotoxic activity and IFN-gamma production (4, 11). IL‑21 R engagement on CD8+ T cells aids control of viral infection and tumor growth; IL-21 R is also necessary for sufficient numbers of regulatory T cells to combat chronic inflammation (1, 12, 13). IL-21 R expression is often upregulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL) (1, 2, 14, 15).

  • References:
    1. Leonard, W.J. et al. (2008) J. Leukoc. Biol. 84:348.
    2. Konforte, D. et al. (2009) J. Immunol. 182:1791.
    3. Monteleone, G. et al., 2009, Cytokine Growth Factor Rev. 20:185.
    4. Parrish-Novak, et al. (2000) Nature 408:57.
    5. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
    6. Asao, H. et al. (2001) J. Immunol. 167:1.
    7. Habib, T. et al. (2002) Biochemistry 41:8725.
    8. Ozaki, K. et al. (2002) Science 298:1630.
    9. Rankin, A.L. et al. (2011) J. Immunol. 186:667.
    10. King, I.L. et al. (2010) J. Immunol. 185:6138.
    11. Kasaian, M.T. et al. (2002) Immunity 16:559.
    12. Frohlich, A. et al. (2009 Science 324:1576.
    13. Tortola, L. et al. (2010) Blood 116:5200.
    14. Jin, H. et al. (2009) J. Clin. Invest. 119:47.
    15. Sarosiek, K.A. et al. (2010) Blood 115:570.
  • Long Name:
    Interleukin 21 Receptor
  • Entrez Gene IDs:
    50615 (Human); 60504 (Mouse)
  • Alternate Names:
    CD360 antigen; CD360; IL-21 receptor; IL21R; IL-21R; interleukin 21 receptor; interleukin-21 receptor; MGC10967; NILR; Novel interleukin receptor
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 2 of 2
Filter your results:

Sample Type
  1. Dual regulation of IRF4 function in T and B cells is required for the coordination of T-B cell interactions and the prevention of autoimmunity.
    Authors: Biswas PS, Gupta S, Stirzaker RA
    J. Exp. Med., 2012;209(3):581-96.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
    Authors: Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
    J. Immunol., 2007;178(9):5957-65.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 PE-conjugated Antibody

Ctrl IC005P  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse IL-21 R PE-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee


Customer Information