Mouse IL-23 R Antibody

(6 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-23 R in Western blots. In Western blots, this antibody does not cross-react with recombinant human IL-23 R.
  • Source
    Monoclonal Rat IgG2B Clone # 258010
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IL-23 R
    Gly24-Asp372
    Accession # Q8K4B4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse IL‑23 R Fc Chimera (Catalog # 1686-MR)
  • Neutralization
    Measured by its ability to neutralize IL‑23-induced IL‑17 secretion in mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 0.75 ng/mL Recombinant Mouse IL‑23 and 10 ng/mL Recombinant Mouse IL‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IL‑17 Secretion Induced by
IL‑23 and Neutralization by Mouse IL‑23 R Antibody.
In the presence of Recombinant Mouse IL‑2 (10 ng/mL, Catalog # 402-ML), Recombinant Mouse IL‑23 (Catalog # 1887-ML) stimulates IL‑17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL‑17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL‑17 secretion elicited by Recombinant Mouse IL‑23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑23 R Monoclonal Antibody (Catalog # MAB1686). The ND50 is typically 0.1-0.4 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-23 R

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1‑5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Mouse IL-23 R cDNA encodes a 644 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 349 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 249 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Mouse IL-23 R is expressed in mouse Th1 and Th2 cells, bone marrow, dendritic cells and macrophages. It is also expressed by mouse CD4+ CD45RBlow memory T cells but at much lower levels by mouse CD4+ CD45RBhigh cells. IL-23 initiates a signal transduction cascade similar to that of IL-12 and involves Jak2, Tyk2, STAT1, STAT3, STAT4, and STAT5. IL-23 has biological activities that are similar to, but distinct from, IL-12.

  • References:
    1. Oppmann, B. et al. (2000) Immunity 13:715.
    2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
    3. Parham, C. et al. (2002) J. Immunol. 168:5448.
    4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
    5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
  • Long Name:
    Interleukin 23 Receptor
  • Entrez Gene IDs:
    149233 (Human); 209590 (Mouse)
  • Alternate Names:
    IBD17; IL-23 R; IL-23 receptor; IL23R; IL-23R; interleukin 23 receptor; interleukin-23 receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Staphylococcus aureus infection of mice expands a population of memory gammadelta T cells that are protective against subsequent infection.
    Authors: Murphy A, O'Keeffe K, Lalor S, Maher B, Mills K, McLoughlin R
    J Immunol, 2014;192(8):3697-708.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  2. IL-22 from conventional NK cells is epithelial regenerative and inflammation protective during influenza infection.
    Authors: Kumar P, Thakar M, Ouyang W, Malarkannan S
    Mucosal Immunol, 2013;6(1):69-82.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy.
    Authors: Ma Y, Aymeric L, Locher C, Mattarollo SR, Delahaye NF, Pereira P, Boucontet L, Apetoh L, Ghiringhelli F, Casares N, Lasarte JJ, Matsuzaki G, Ikuta K, Ryffel B, Benlagha K, Tesniere A, Ibrahim N, Dechanet-Merville J, Chaput N, Smyth MJ, Kroemer G, Zitvogel L
    J. Exp. Med., 2011;208(3):491-503.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  4. A human colonic commensal promotes colon tumorigenesis via activation of T helper type 17 T cell responses.
    Authors: Wu S, Rhee KJ, Albesiano E, Rabizadeh S, Wu X, Yen HR, Huso DL, Brancati FL, Wick E, McAllister F, Housseau F, Pardoll DM, Sears CL
    Nat. Med., 2009;15(9):1016-22.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  5. Resident Vdelta1+ gammadelta T cells control early infiltration of neutrophils after Escherichia coli infection via IL-17 production.
    Authors: Shibata K, Yamada H, Hara H, Kishihara K, Yoshikai Y
    J. Immunol., 2007;178(7):4466-72.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  6. Cutting edge: An in vivo requirement for STAT3 signaling in TH17 development and TH17-dependent autoimmunity.
    Authors: Harris TJ, Grosso JF, Yen HR, Xin H, Kortylewski M, Albesiano E, Hipkiss EL, Getnet D, Goldberg MV, Maris CH, Housseau F, Yu H, Pardoll DM, Drake CG
    J. Immunol., 2007;179(7):4313-7.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
Isotype Controls
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