Mouse IL-27/IL-35 EBI3 Subunit APC-conjugated Antibody

(2 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-27/IL-35 EBI3 Subunit in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human IL‑27/IL-35 EBI3 subunit.
  • Source
    Monoclonal Rat IgG2A Clone # 355022
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IL‑27 heterodimer
    Tyr19-Pro228 (EBI3) & Phe29-Ser234 (p28)
    Accession # O35228 (EBI3) & Q8K3I6 (p28)
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL-27/IL-35 EBI3 Subunit in Mouse splenocytes by Flow Cytometry. Mouse splenocytes untreated (open histogram) or treated with LPS (filled histogram) were stained with Rat Anti-Mouse IL-27/IL-35 EBI3 Subunit APC-conjugated Monoclonal Antibody (Catalog # IC18341A) or isotype control antibody (Catalog # IC006A, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-27/IL-35 EBI3 Subunit
IL-27 is a heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus-induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naive CD4+ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL-12-mediated Th1 cell development. Human EBI3 also associates with the p35 subunit of IL-12 to form IL-35 which is important for immunosuppressive Treg cell induction. EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide anda 209 aa mature protein with two fibronectin type III domains.
  • Long Name:
    Epstein-Barr virus induced gene 3
  • Entrez Gene IDs:
    10148 (Human); 50498 (Mouse); 680609 (Rat)
  • Alternate Names:
    cytokine receptor; EBI3; EBV-induced gene 3 protein; Epstein-Barr virus induced 3; Epstein-Barr virus induced gene 3; Epstein-Barr virus-induced gene 3 protein; IL-27 subunit beta; IL-27/IL-35 EBI3 Subunit; IL27B; IL-27B; IL35 EBI3; interleukin-27 subunit beta
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Allophycocyanin Isotype Control

Ctrl IC006A 5  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 1
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Average Rating: 4.5 (Based on 2 reviews)

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We have 2 reviews tested in 1 species: Mouse.
We have 2 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Mouse IL-27/IL-35 EBI3 Subunit APC-conjugated Antibody IC18341A
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Excellent
 Flow Mouse Anonymous 04/13/2017
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Image Details
Flow Cytometry Mouse IL-27/IL-35 EBI3 Subunit APC-conjugated Antibody IC18341A
Flow Cytometry: Mouse IL-27/IL-35 EBI3 Subunit APC-conjugated Antibody [IC18341A]

Summary

ApplicationFlow Cytometry
Sample TestedMouse splenocytes
SpeciesMouse
  Very Good
 Flow Mouse Anonymous 07/27/2016
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Summary

ApplicationFlow Cytometry
Sample TestedSplenic T cells
SpeciesMouse

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