Mouse IL‑27/IL‑35 EBI3 Subunit PE‑conjugated Antibody

  • Specificity
    Detects mouse IL-27/IL-35 EBI3 Subunit in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human IL‑27/IL-35 EBI3 subunit.
  • Source
    Monoclonal Rat IgG2A Clone # 355022
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IL‑27 heterodimer
    Tyr19-Pro228 (EBI3) & Phe29-Ser234 (p28)
    Accession # O35228 (EBI3) & Q8K3I6 (p28)
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL-27/IL-35 EBI3 Subunit in Mouse splenocytes by Flow Cytometry. Mouse splenocytes untreated (open histogram) or treated with LPS (filled histogram) were stained with Rat Anti-Mouse IL-27/IL-35 EBI3 Subunit PE-conjugated Monoclonal Antibody (Catalog # IC18341A) or isotype control antibody (Catalog # IC006P, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-27/IL-35 EBI3 Subunit
IL-27 is a heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus-induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naive CD4+ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL-12-mediated Th1 cell development. Human EBI3 also associates with the p35 subunit of IL-12 to form IL-35 which is important for immunosuppressive Treg cell induction. EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide anda 209 aa mature protein with two fibronectin type III domains.
  • Long Name:
    Epstein-Barr virus induced gene 3
  • Alternate Names:
    EBI3; IL-27B; cytokine receptor; EBV-induced gene 3 protein; Epstein-Barr virus induced 3; Epstein-Barr virus induced gene 3; Epstein-Barr virus-induced gene 3 protein; IL-27 subunit beta; IL27B; IL-27B; interleukin-27 subunit beta
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