Mouse IL-3 R beta PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse IL‑3 R beta in direct ELISAs. Cross-reactivity with AIC2B was not tested.
  • Source
    Monoclonal Rat IgG2A Clone # 130705
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IL-3 R beta
    Accession # P26954
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑3 R beta in DA3 Mouse Cell Line by Flow Cytometry. DA3 mouse myeloma cell line was stained with Rat Anti-Mouse IL‑3 R beta PE-conjugated Monoclonal Antibody (Catalog # FAB5492P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-3 R beta

Interleukin 3 (IL-3) is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta  subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta  subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta. AIC2A was found to be the result of a gene duplication event. Both the alpha  and the beta  subunits are members of the cytokine receptor superfamily (3).

  • References:
    1. Itoh, N. et al. (1990) Science 247:324.
    2. Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.
    3. Schrader, J.W. in Cytokine Reference, (2001) J.J. Oppenheim and M. Feldmann, eds. Academic Press, New York, p. 1899.
  • Long Name:
    Interleukin 3 Receptor beta
  • Entrez Gene IDs:
    12984 (Mouse)
  • Alternate Names:
    AIC2A; BetaIl3; Bil3; Csfgmrb; IL3R beta; IL3R; IL3RB; IL-3Rb; Il3rb2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 13  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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