Mouse IL-5 PE-conjugated Antibody

IC405P has been discontinued. View all IL-5 products.
(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects human and mouse IL-5 in direct ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) IL-3, IL-5, rhGM-CSF, recombinant mouse (rm) IL-3 or rmGM-CSF is observed.
  • Source
    Monoclonal Rat IgG1 Clone # TRFK5
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse T cell clone-derived partially purified recombinant mouse IL-5
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑5 in EL‑4 Mouse Cell Line by Flow Cytometry. EL‑4 mouse lymphoblast cell line was stained with Rat Anti-Mouse IL‑5 PE‑conjugated Monoclonal Antibody (Catalog # IC405P, filled histogram) or with primary antibody omitted (open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-5

IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.

  • Long Name:
    Interleukin 5
  • Entrez Gene IDs:
    3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
  • Alternate Names:
    BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Multiplex immunoassay characterization and species comparison of inflammation in acute and non-acute ischemic infarcts in human and mouse brain tissue
    Acta Neuropathol Commun, 2016;4(1):100.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow

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