Detection of Integrin alpha 4/CD49d in B16‑F1 Mouse Cell Line by Flow Cytometry. B16‑F1 mouse melanoma cell line was stained with Rat Anti-Mouse Integrin alpha 4/CD49d APC‑conjugated Monoclonal Antibody (Catalog # FAB2450A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha 4/CD49d
Integrin alpha 4, also known as CD49d and VLA sunbunit alpha, associates with either Integrin beta 1 or beta 7 subunits to form heterodimers that serve as receptors for fibronectin and VCAM-1. Integrin alpha 4 beta 1 is expressed on peripheral lymphocytes, thymocytes, and monocytes, while Integrin alpha 4 beta 7 is expressed on peripheral lymphocytes and a small subset of thymocytes. On T cells, the alpha 4 subunit may contribute to cytotoxic activity. Over amino acids 34-976, mouse Integrin alpha 4 shares 85% amino acid sequence identity to human Integrin alpha 4.
Entrez Gene IDs:
3676 (Human); 16401 (Mouse)
269C wild type; CD49 antigen-like family member D; CD49d antigen; CD49d; CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor; IA4; Integrin alpha 4; integrin alpha-4 subunit; integrin alpha-4; Integrin alpha-IV; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); ITGA4; MGC90518; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha; VLA-4
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