Mouse Integrin  alpha 9 Antibody

Catalog # Availability Size / Price Qty
AF3827
AF3827-SP
Integrin alpha 9 in Mouse Liver Tissue.
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Product Details
Citations (22)
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Mouse Integrin  alpha 9 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha 9 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant human Integrin alpha 9 is observed and less than 5% cross-reactivity with recombinant mouse Integrin alpha 3 and alpha 4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Integin alpha 9
Try31-Val979
Accession # NP_598482
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Integrin  alpha 9
Flow Cytometry
2.5 µg/106 cells
D3 mouse embryonic stem cell line
Immunohistochemistry
1-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Integrin alpha 9 antibody in Mouse Liver Tissue by Immunohistochemistry (IHC-Fr). View Larger

Integrin alpha 9 in Mouse Liver Tissue. Integrin a9 was detected in perfusion fixed frozen sections of mouse liver tissue using Goat Anti-Mouse Integrin a9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3827) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to bile canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry/ Immunofluorescence Detection of Human Integrin alpha 9 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Integrin alpha 9 by Immunocytochemistry/Immunofluorescence Morphology and protein expression of hSKPs. (a) SEM micrographs of nanofibrous scaffolds showing morphology of hSKPs at 7 days of culture. (b) Immunofluorescent images of hSKPs grown on nanofibrous scaffolds showing expression of integrin alpha -9 (green), fibronectin (red) and procollagen I & III (green). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28860484), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Integrin alpha 9 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Integrin alpha 9 by Immunocytochemistry/Immunofluorescence Intergins alpha 4/ alpha 9 are responsible for the action of osteopontin (OPN) in the augmentation of macrophage (Mφ) M2 polarization and angiogenic capacity. (A) Relative mean fluorescence intensities (MFIs) of integrins alpha 4/ alpha 9 were measured by FACS analysis in each Mφ subset. Anti-integrin alpha 4 or alpha 9 antibodies (Abs) and each isotype-matched control Ab were used at 10 µg/mL, n = 4 [***p < 0.001 vs. untreated, ###p < 0.001 vs. interleukin (IL)-10 alone]. (B) Representative confocal laser scanning immunofluorescence overlay images of integrin alpha 4 (green) and DAPI (blue), as well as those of integrin alpha 9 (red) and DAPI (blue) in Mφ (–) and Mφ (IL-10 + IL-18). Scale bar represents 20 µm. (C) Relative MFI of CD163 was measured by FACS analysis in each Mφ subset. Anti-integrin alpha 4 or alpha 9 Abs and each isotype-matched control Ab were used at 10 µg/mL, n = 4 (***p < 0.001 vs. untreated, ###p < 0.001 vs. IL-10 alone, †††p < 0.001 vs. IL-10 + IL-18). (D) The total areas and lengths of tube-like structures were determined by the Matrigel tube formation assay where b.End5 was cocultured with each Mφ subset. Anti-integrin alpha 4 or alpha 9 Abs and each isotype-matched control Ab were used at 10 µg/mL, n = 6 (***p < 0.001, **p < 0.01, *p < 0.05 vs. untreated, ###p < 0.001, ##p < 0.01, #p < 0.05 vs. IL-10 alone, †††p < 0.001 vs. IL-10 + IL-18). Integrin alpha 4 = ITGA4; Integrin alpha 9 = ITGA9. All data are expressed as means ± SEM and were analyzed by a one-way ANOVA followed by Tukey’s test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29559970), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 9

Integrin alpha 9 is a 150 kDa type I transmembrane glycoprotein that is widely expressed and is found on smooth muscle, keratinocytes, skeletal muscle and hepatocytes. It principally associates with the beta 1 integrin. alpha 9 beta 1 binds to VEGF-C, VEGF-D, and osteopontin. The 951 amino acid (aa) extracellular domain of mouse integrin alpha 9 contains three beta -propellor repeats and multiple PheGly-GlyAlaPro repeats. A potential proteolytic cleavage site in human alpha 9 ECD is absolutely conserved in mouse alpha 9 ECD (Arg566-Val567). The ECD of mouse integrin alpha 9 shares 95% and 89% aa sequence identity to rat and human integrin alpha 9, respectively.

Entrez Gene IDs
3680 (Human); 104099 (Mouse); 685004 (Rat)
Alternate Names
ALPHA-RLC; Integrin alpha 9; integrin alpha-9; Integrin alpha-RLC; integrin, alpha 9; ITGA4L; ITGA9; RLC; RLC-alpha

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Citations for Mouse Integrin  alpha 9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Organ function is preserved despite reorganization of niche architecture in the hair follicle
    Authors: Wei, H;Du, S;Parksong, J;Pasolli, HA;Matte-Martone, C;Regot, S;Gonzalez, LE;Xin, T;Greco, V;
    Cell stem cell
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Progenitor-derived endothelin controls dermal sheath contraction for hair follicle regression
    Authors: P Martino, R Sunkara, N Heitman, M Rangl, A Brown, N Saxena, L Grisanti, D Kohan, M Yanagisawa, M Rendl
    Nature Cell Biology, 2023-01-30;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing
    Authors: S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie
    Cell Stem Cell, 2020-08-04;27(3):396-412.e6.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Mechanically activated ion channel PIEZO1 is required for lymphatic valve formation
    Authors: K Nonomura, V Lukacs, DT Sweet, LM Goddard, A Kanie, T Whitwam, SS Ranade, T Fujimori, ML Kahn, A Patapoutia
    Proc. Natl. Acad. Sci. U.S.A., 2018-11-27;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis
    Authors: T Kobori, S Hamasaki, A Kitaura, Y Yamazaki, T Nishinaka, A Niwa, S Nakao, H Wake, S Mori, T Yoshino, M Nishibori, H Takahashi
    Front Immunol, 2018-03-06;9(0):334.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  6. Biocomposite nanofiber matrices to support ECM remodeling by human dermal progenitors and enhanced wound closure
    Authors: F Anjum, NA Agabalyan, HD Sparks, NL Rosin, MS Kallos, J Biernaskie
    Sci Rep, 2017-08-31;7(1):10291.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Defective lymphatic valve development and chylothorax in mice with a lymphatic-specific deletion of Connexin43
    Authors: Alexander M Simon
    Dev. Biol., 2016-11-27;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Integrin Alpha 9 Blockade Suppresses Lymphatic Valve Formation and Promotes Transplant Survival
    Invest. Ophthalmol. Vis. Sci., 2016-11-01;57(14):5935-5939.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development.
    Authors: Geng X, Cha B, Mahamud M, Lim K, Silasi-Mansat R, Uddin M, Miura N, Xia L, Simon A, Engel J, Chen H, Lupu F, Srinivasan R
    Dev Biol, 2015-11-02;409(1):218-33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Kindlin-3-mediated integrin adhesion is dispensable for quiescent but essential for activated hematopoietic stem cells.
    Authors: Ruppert R, Moser M, Sperandio M, Rognoni E, Orban M, Liu W, Schulz A, Oostendorp R, Massberg S, Fassler R
    J Exp Med, 2015-08-17;212(9):1415-32.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Structural and functional features of central nervous system lymphatic vessels.
    Authors: Louveau A, Smirnov I, Keyes T, Eccles J, Rouhani S, Peske J, Derecki N, Castle D, Mandell J, Lee K, Harris T, Kipnis J
    Nature, 2015-06-01;523(7560):337-41.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity.
    Authors: Park D, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong K, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong Y, Koh G
    J Clin Invest, 2014-07-25;124(9):3960-74.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. The Schlemm's canal is a VEGF-C/VEGFR-3-responsive lymphatic-like vessel.
    Authors: Aspelund A, Tammela T, Antila S, Nurmi H, Leppanen V, Zarkada G, Stanczuk L, Francois M, Makinen T, Saharinen P, Immonen I, Alitalo K
    J Clin Invest, 2014-07-25;124(9):3975-86.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  14. Angiopoietin 2 regulates the transformation and integrity of lymphatic endothelial cell junctions.
    Authors: Zheng W, Nurmi H, Appak S, Sabine A, Bovay E, Korhonen E, Orsenigo F, Lohela M, D'Amico G, Holopainen T, Leow C, Dejana E, Petrova T, Augustin H, Alitalo K
    Genes Dev, 2014-07-15;28(14):1592-603.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Corneal lymphatic valve formation in relation to lymphangiogenesis.
    Authors: Truong, Tan, Huang, Eric, Yuen, Don, Chen, Lu
    Invest Ophthalmol Vis Sci, 2014-03-25;55(3):1876-83.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Smooth muscle-endothelial cell communication activates Reelin signaling and regulates lymphatic vessel formation.
    J. Cell Biol., 2012-06-04;197(6):837-49.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Polydom/SVEP1 Is a Ligand for Integrin alpha9beta1.
    Authors: Sato-Nishiuchi R, Nakano I, Ozawa A, Sato Y, Takeichi M, Kiyozumi D, Yamazaki K, Yasunaga T, Futaki S, Sekiguchi K
    J. Biol. Chem., 2012-05-31;287(30):25615-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  18. Extracellular matrix protein tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration.
    Authors: Nakamura-Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, Nagasawa T, Suda T, Kubota Y
    Blood, 2012-05-02;119(23):5429-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Genes regulating lymphangiogenesis control venous valve formation and maintenance in mice.
    Authors: Bazigou E, Lyons OT, Smith A, Venn GE, Cope C, Brown NA, Makinen T
    J. Clin. Invest., 2011-07-18;121(8):2984-92.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  20. Single transcription factor reprogramming of hair follicle dermal papilla cells to induced pluripotent stem cells.
    Authors: Tsai SY, Bouwman BA, Ang YS, Kim SJ, Lee DF, Lemischka IR, Rendl M
    Stem Cells, 2011-06-01;29(6):964-71.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Oct4 and klf4 reprogram dermal papilla cells into induced pluripotent stem cells.
    Authors: Tsai SY, Clavel C, Kim S, Ang YS, Grisanti L, Lee DF, Kelley K, Rendl M
    Stem Cells, 2010-02-01;28(2):221-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  22. Increased osteopontin expression in dendritic cells amplifies IL-17 production by CD4+ T cells in experimental autoimmune encephalomyelitis and in multiple sclerosis.
    Authors: Murugaiyan G, Mittal A, Weiner HL
    J. Immunol., 2008-12-01;181(11):7480-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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