Mouse Integrin alpha 9 Antibody

Integrin alpha 9 in Mouse Liver Tissue.

Integrin a9 was detected in perfusion fixed frozen sections of mouse liver tissue using Goat Anti-Mouse Integrin a9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3827) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to bile canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human Integrin alpha 9 by Immunocytochemistry/Immunofluorescence

Morphology and protein expression of hSKPs. (a) SEM micrographs of nanofibrous scaffolds showing morphology of hSKPs at 7 days of culture. (b) Immunofluorescent images of hSKPs grown on nanofibrous scaffolds showing expression of integrin alpha -9 (green), fibronectin (red) and procollagen I & III (green). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28860484), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Integrin alpha 9 by Immunocytochemistry/Immunofluorescence

Intergins alpha 4/ alpha 9 are responsible for the action of osteopontin (OPN) in the augmentation of macrophage (Mφ) M2 polarization and angiogenic capacity. (A) Relative mean fluorescence intensities (MFIs) of integrins alpha 4/ alpha 9 were measured by FACS analysis in each Mφ subset. Anti-integrin alpha 4 or alpha 9 antibodies (Abs) and each isotype-matched control Ab were used at 10 µg/mL, n = 4 [***p < 0.001 vs. untreated, ###p < 0.001 vs. interleukin (IL)-10 alone]. (B) Representative confocal laser scanning immunofluorescence overlay images of integrin alpha 4 (green) and DAPI (blue), as well as those of integrin alpha 9 (red) and DAPI (blue) in Mφ (–) and Mφ (IL-10 + IL-18). Scale bar represents 20 µm. (C) Relative MFI of CD163 was measured by FACS analysis in each Mφ subset. Anti-integrin alpha 4 or alpha 9 Abs and each isotype-matched control Ab were used at 10 µg/mL, n = 4 (***p < 0.001 vs. untreated, ###p < 0.001 vs. IL-10 alone, †††p < 0.001 vs. IL-10 + IL-18). (D) The total areas and lengths of tube-like structures were determined by the Matrigel tube formation assay where b.End5 was cocultured with each Mφ subset. Anti-integrin alpha 4 or alpha 9 Abs and each isotype-matched control Ab were used at 10 µg/mL, n = 6 (***p < 0.001, **p < 0.01, *p < 0.05 vs. untreated, ###p < 0.001, ##p < 0.01, #p < 0.05 vs. IL-10 alone, †††p < 0.001 vs. IL-10 + IL-18). Integrin alpha 4 = ITGA4; Integrin alpha 9 = ITGA9. All data are expressed as means ± SEM and were analyzed by a one-way ANOVA followed by Tukey’s test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29559970), licensed under a CC-BY license. Not internally tested by R&D Systems.