Detects mouse Integrin beta 1/CD29 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta 1, recombinant mouse (rm) Integrin beta 2, rmIntegrin beta 3, rmIntegrin beta 4, or rmIntegrin beta 6 is observed.
Monoclonal Rat IgG2A Clone # 265917
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 1/CD29 Gln21-Asp728 Accession # P09055
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Integrin beta 1/CD29 in B16‑F1 Mouse Cell Line by Flow Cytometry. B16‑F1 mouse melanoma cell line was stained with Rat Anti-Mouse Integrin beta 1/CD29 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB2405F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin beta 1/CD29
The Integrin beta 1 subunit, also known as CD29 and VLA-4 beta, is a 128-132 kDa type I transmembrane glycoprotein member of the integrin beta -chain family of molecules. It associates with multiple distinct Integrin alpha subunits. The Integrin beta 1 subunit has a broad tissue distribution with the exception of erythrocytes. Over amino acids 21-728, mouse Integrin beta 1 shares 99% and 92% amino acid sequence identity with rat and human Integrin beta 1, respectively.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.